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. 2018 Apr 24;10(5):216. doi: 10.3390/v10050216

Figure 7.

Figure 7

Viral infection and GUS activity in LIYV-L2CGUS inoculated Hc-Pro Nicotiana benthamiana plants. (A) Detection of viral infection and insertion integrity by RT-PCR with total RNA extracted from upper non-inoculated leaves at 3 wpi and 5 wpi. Two primer sets were used to amplify the sequence of LIYV CP (CP, 530 bp) and the sequence flanking the GUS cassette (Insert, 2279 bp), LIYV-WT without the insert was amplified as a control (317 bp). The bands in between indicate partial deletions of the GUS cassette. (B) Systemic spread and distribution of GUS activity monitored in intact leaves and the cross-sections of petioles and shoots of LIYV-L2CGUS infected plants at 5 wpi. Blue stain indicates GUS activity and was detected only in the vascular tissues. Tissues from LIYV-WT infected plants are applied as a negative control.