Figure 2.

Measurement of the autophagic flux in ZIKV-infected HUVEC. (A) Western blot analysis of p62 degradation. The time course of expression of p62 in ZIKV-infected HUVEC at MOI 1 was investigated using anti-p62 antibody. Mock-infected HUVEC were used as negative controls, rapamycin (100 nM) treatment was used as a positive control, and GAPDH was used as a protein-loading control; (B) the ratios of P62 to GAPDH were calculated, and representative results are presented with graphs. Data are presented as means from three independent experiments. Compared to the control group, significance is analyzed with two-tailed Student’s t test. * p < 0.05; ** p < 0.01. (C) HUVEC transduced with lentivirus expressing mTagRFP-mWasabi-LC3 were either uninfected or infected with ZIKV at MOI 1. The cells were fixed and visualized by confocal microscopy at 18 and 24 h post-infection, respectively. Bars, 10 µm.