Figure 3.
Conventional dendritic cells (cDCs) are the main target for intravenously (i.v.) injected modified vaccinia virus Ankara (MVA). C57BL/6 mice were immunized i.v. with 5 × 107 TCID 50 recombinant MVA (rMVA) or rMVA‐CD70. Naive mice served as control. Splenic antigen‐presenting cell (APC) subsets were analysed after 6, 24 and 48 hr by 12‐colour flow cytometry as shown in Fig. 1(a). Bar graphs show the mean fluorescence intensity (MFI) ± SEM of CD86 and CD40 and the relative MFI (rMFI) for CD70. The rMFI was calculated by subtracting the MFI of the fluorescence‐minus‐one control from the MFI of stained samples. Data are from three mice per group and time point.