Figure 9. P2Y₂ dependent signaling is conserved in breast cancer cells.

MCF10A, MDA-MB-231 and MDA-MB-436 cells were treated with a selective and competitive P2Y₂ receptor antagonist (AR-C 118925XX) in order to test if scratch-induced signaling to neighboring cells was dependent on P2Y₂. (A) Both MCF10A and MDA-MB-231 cells show a significant reduction in signaling to neighbors compared with control (signaling to neighbors in absence of AR-C 118925XX), suggesting a similar mechanism. Data show no significant difference in neighboring cells peak ΔF/F between control and P2Y₂ inhibition for MDA-MB-436, however this is likely due to a lack of signaling to neighbors at baseline (control). (B) Kymographs (ROI-independent analysis) highlight inhibition of scratch-induced signaling to neighbors with selective P2Y₂ antagonist. Data presented as mean ± standard deviation. ^*indicates significance, P < 0.05 via paired t-test. N.S. indicates no significant difference. Data represent MCF10A (N=10 control and N=10 inhibitor), 231 (N=10 control and N=10 inhibitor), 436 (N=15 control and N=10 inhibitor) in total from 2 independent experiments for each group. Control data from Figure 8 was used for comparison here.