Table 3.
Acquisition parameters and observed spectral features for 2D heteronuclear correlation experiments performed on 2H/15N N130.
| Experiment | 1H-TROSY | 15N-TROSY |
|---|---|---|
| Points (F2 x F1) | 1024 x 218 | 1024 x 136 |
| Acquisition time (ms) | 45 x 42 | 198 x 14 |
| Scans | 64 | 96 |
| Recycle Delay (s) | 1 | 1 |
| Exp Time (hrs:min) | 4:18 | 4:31 |
| <FWHH 1HN> pentamer (Hz) | 37.8 ± 3.2 (n = 20) | 43.2 ± 3.9 (n = 20) |
| <FWHH 1HN> IDR (Hz) | 31.4 ± 1.9 (n = 8) | 39.1 ± 2.2 (n = 8) |
| <FWHH 15NH> pentamer (Hz) | 22.9 ± 2.6 (n = 20) | 15.2 ± 2.4 (n = 20) |
| <FWHH 15NH> IDR (Hz) | 17.4 ± 2.6 (n = 8) | 11.6 ± 1.7 (n = 8) |
| Signal-to-noise | 115 | 40 |
| # peaks (observed/possible) | 124/124 | 124/124 |