Abstract
Background
The aim was to analyze hepatic hypertrophy after portal vein embolization (PVE) and Associating Liver Partition with Portal vein ligation for Staged hepatectomy (ALPPS) to determine whether clinical circumstances associated with major hepatic resections correlated with remnant growth.
Methods
Data was abstracted from a retrospectively maintained database on 27 patients undergoing hepatic resection followed by PVE and the ALPPS procedure between October 1, 2007 and December 31, 2016. The increasing rate of liver volume and remnant liver LU15 was defined as the percentage-point difference between the liver volume and remnant liver LU15 before and after the intervention or surgery. And correlation between kinetic growth rate (KGR) of liver and future remnant liver volume or remnant liver LU15 was analyzed.
Results
The degree of hypertrophy (DH) of volume and LU15 was significantly greater after ALPPS (volume: 40.3% and LU15: 65.0%) than after PVE (volume: 22.7% and LU15: 48.8%) (P < 0.05). KGR of volume and LU15 was significantly greater after ALPPS (volume: 19.0 cm3/day and 2.00%/day) (LU15: 0.61 /day and 1.82%/day) than after PVE (volume: 3.89 cm3/day and 0.42%/day) (LU15: 0.19 /day and 0.63%/day) (P < 0.001). An inverse correlation between KGR and initial remnant liver volume was observed. And a positive correlation between KGR and LU15 was observed.
Conclusion
Future remnant liver volume and KGR was greater after the ALPPS procedure than after PVE. Liver hypertrophy is related to the expected remnant liver volume and total liver function. This study suggested that total liver function and initial remnant liver volume might be a new indication of hepatectomy after PVE and ALPPS in the case of insufficient remnant liver volume.
Keywords: Liver kinetic growth, 99mTc-galactosyl human serum albumin scintigraphy, Remnant liver function
Background
Hepatic resection is a potentially curative treatment for a variety of primary hepatobiliary malignancies as well as for numerous metastatic malignancies. As more extensive hepatic resections are performed, achieving adequate remnant liver function often remains the rate limiting step [1]. Assessment of hepatic functional reserve is one of the most important steps in hepatic resection [2].
To estimate hepatic functional reserve, 99mTc-labelled diethylene triamine penta-acetate–galactosyl human serum albumin (99mTc-GSA), a radiopharmaceutical that binds specifically to the hepatic asialoglycoprotein receptor (ASGP-R), has been developed and used clinically to estimate hepatic function [3]. Because ASGP-R is a natural superficial antigen of viable hepatocytes, the uptake of 99mTc-GSA is independent of biochemical processes and allows direct estimation of the functioning hepatocyte mass [4]. In addition, the distribution of 99mTc-GSA in the liver is not dependent on liver blood flow [5]. Our previous study has shown that a novel index, remnant liver LU15, has been identified as a surrogate marker for remnant liver function and preoperative remnant liver LU15 values might predict hepatic failure following a liver resection without biliary reconstruction [6].
Portal vein embolization (PVE) has been repeatedly shown as a reliable technique to induce atrophy of the embolized lobe and compensatory hypertrophy of the future remnant liver, and it currently remains the standard for achieving an appropriate future remnant liver before hepatic resection [7]. The kinetic growth rate (KGR) of future remnant liver after PVE has been reported to be 2.4% per week on average, achieving an increase in future remnant liver from 10 to 46% from 2 to 8 weeks post PVE, respectively [8].
Recently a novel operative approach, the ALPPS procedure (Associating Liver Partition with Portal vein ligation for Staged hepatectomy), has been used to induce hypertrophy of the future liver remnant (FLR) and expedite stages of hepatic resections.
In fact, data show that the standardized future remnant liver has grown by 40–160% in only 6–9 days after ALPPS [9–11]. The mechanisms of the apparent profound hepatic growth of future remnant liver after ALPPS are unknown.
To date, no study has compared the degree and rate of growth of future remnant liver in patients after PVE and ALPPS using a surrogate parameter, remnant liver LU15, by using fusions obtained from contrast-enhanced computed tomography (CT) and 99mTc-GSA single photon emission CT (SPECT). The aim of the present study was to compare the degree and rate of hepatic hypertrophy after PVE and ALPPS to determine whether clinical circumstances associated with major hepatic resections correlated with remnant growth.
Methods
The retrospective data collection in this study was performed with approval of the Tokyo Medical University Hachioji Medical Center Ethics Committee. Data were abstracted from a retrospectively maintained database on all 27 patients undergoing hepatic resection followed by PVE and the ALPPS procedure between October 1, 2007 and Dec 31, 2016. The indication of hepatectomy for liver metastasis was good function of future remnant liver and without extrahepatic lesions. And the PVE indication was less than 13.0 of remnant liver LU calculated by 99 m-Tc-GSA liver scintigrahy [6]. ALLPS procedure was performed with only the patients, who has insufficient future remnant liver volume and whom was planned surgical procedure of tri-sectionectomy and partial hepatectomy.
All patients with available volumetric data and parameters from 99mTc-GSA liver scintigraphy were included. As part of our standard protocols, patients underwent a CT scan, along with hepatic volumetry and 99mTc-GSA liver scintigraphy with several parameters including remnant liver LU15, before the ALPPS procedure, and 4 days and 7 days after the ALPPS procedure. Total liver LU15 was calculated from the cumulative liver uptake of the tracer 15 to 16 min after an injection of a radiotracer. Remnant liver LU15 was calculated as an index or residual liver function by applying the following equation; Remnant liver LU15 = Total LU15 x residual count ratio (calculated by fusion image of the SPECT images by scintigraphy and a three-dimensional (3D) image of the liver constructed by Synapse Vinscent (Fuji Film, Tokyo, JAPAN)). CT volumetry was performed in patients undergoing PVE immediately prior to embolization and at 3–4 weeks after a PVE just prior to a major hepatic resection. PVE with resected planning branches was used.
Liver volumes were determined by loading CT images onto a Synapse Vincent. The increasing rate of liver volume and remnant liver LU15 was defined as the percentage-point difference between the liver volume and remnant liver LU15 before and after the intervention or surgery (PVE or ALPPS). KGR was calculated as percentage growth per day. [12]
In patients who underwent liver resection, post-operative liver failure was determined according to the International Study Group of Liver Surgery (ISGLS) classification [13]. In this study, grade B and C was defined as postoperative liver failure.Primary end point in this study was the degree of hypertrophy of liver volume and LU15 and correlation of KGR and liver volume or remnant liver LU15.
The degree of hypertrophy of volume and LU15 was significantly greater after ALPPS than after PVE. KGR of volume and LU15 was significantly greater after ALPPS than after PVE. An inverse correlation between KGR and initial remnant liver volume was suggested. And a positive correlation between KGR and LU15 was observed.
All statistical analyses were performed using SPSS package (SPSS 18.0). Differences between groups were analyzed using the unpaired t-test for continuous variables and by the χ2-test or continuity correction method for categorical variables. The Wilcoxon’s rank-sum test was used for variables that did not display a normal distribution. All statistical tests were two-sided, and differences were considered significant when P < 0.05.
Results
We identified 27 patients who underwent PVE and major hepatic resection or the first part of a staged resection and 2 patients who underwent ALPPS procedures. Patient demographics for the two groups are shown in Table 1. The mean age in the PVE group was 69 years and in the ALPPS group was 68 years. Colorectal liver metastasis (CRLM) was the dominant diagnoses for ALPPS group patients, and none of the patients in the ALPPS group had underlying cirrhosis. The PVE group included cholangiocarcionoma (n = 10), hepatocellular carcinoma (HCC; n = 9), CRLM (n = 5) and other disease (n = 3). The surgical procedures in the PVE group were hemi-hepatectomy (n = 22) and tri-sectionectomy (n = 4), which include with pylorus-preserving pancreaticoduodenectomy (PPPD; n = 2), bile-duct reconstruction (n = 5) and vascular reconstruction (n = 5).
Table 1.
Patients demographics
| PVE (n = 27) | ALPPS (n = 2) | |
|---|---|---|
| Age (years) | Median 69 | Median 68 |
| Gender (male) | 19 (70%) | 0 (0%) |
| Diagnosis | ||
| CRLM | 5 (19%) | 2 (100%) |
| Cholangiocarcinoma | 10 (37%) | 0 (0%) |
| HCC | 9 (33%) | 0 (0%) |
| Others | 3 (11%) | 0 (0%) |
| Surgical procedure | ||
| Hr1 | 1 (4%) | 0 (0%) |
| Hr2 | 22 (81%) | 0 (0%) |
| Hr3 | 4 (15%) | 0 (0%) |
| ALPPS | 0 | 2 (100%) |
| with PPPD | 2 (7%) | 0 (0%) |
| with bile-duct reconstruction | 10 (37%) | 0 (0%) |
| with vascular reconstruction | 5 (19%) | 0 (0%) |
| Liver failure | 2 (7%) | 0 (0%) |
| 90 day mortality | 0 (0%) | 0 (0%) |
CRLM Colorectal liver metastasis, HCC Hepatocellular carcinoma, PPPD Pylorus-preserving pancreaticoduodenectomy
Table 2 summarizes volumetric measurements and LU15 values by 99mTc-GSA liver scintigraphy of remnant liver volume before and after PVE and ALLPPS. Growth of the remnant liver volume and remnant liver LU15 are shown in Fig. 1. The degree of hypertrophy (DH) of volume and LU15 was significantly greater after ALPPS (volume: 40.3% and LU15: 65.0%) than after PVE (volume: 22.7% and LU15: 48.8%) (P < 0.05). KGR of volume and LU15 was significantly greater after ALPPS (volume: 19.0 cm3/day and 2.00%/day) (LU15: 0.61 /day and 1.82%/day) than after PVE (volume: 3.89 cm3/day and 0.42%/day) (LU15: 0.19 /day and 0.63%/day) (P < 0.001).
Table 2.
Future remnant liver growth
| PVE (n = 27) | ALPPS (n = 2) | |
|---|---|---|
| Total liver volume (post - pre) (cc) | − 73 | − 91 |
| Remnant liver volume (post - pre) (cc) | 100 | 124 |
| LHL15 (post - pre) | 0.0055 | −0.001 |
| HH15 (post - pre) | 0.006 | 0 |
| LU15 (post - pre) | 0.8 | −0.94 |
| Remnant liver LU15 (post - pre) | 5.75 | 6.28 |
| Time interval (days) | 31 | 10 |
| Remnant liver volume DH (%) | 22.7 | 40.3 |
| Remnant liver LU15 DH (%) | 48.8 | 65.0 |
| KGR (cc/day) | 3.89 | 19.0 |
| KGR (%/day) | 0.42 | 2.00 |
| Remnant liver LU15 KGR (/day) | 0.19 | 0.61 |
| Remnant liver LU15 KGR (%/day) | 0.63 | 1.82 |
All values are median; DH degree hypertrophy, KGR kinetic liver growth rate
Fig. 1.
Transition of kinetic liver growth factors determined by volumetry and scintigraphy prior to and after intervention or surgery in two groups. The degree of hypertrophy (DH) of volume and LU15 was significantly greater after ALPPS (volume: 40.3% and LU15: 65.0%) than after PVE (volume: 22.7% and LU15: 48.8%) (P < 0.05)
KGR (cc/day or %/day) of liver volume and LU15 or pre-operative remnant liver volume was plotted for all patients (Fig. 2). An inverse correlation between KGR of liver volume and initial remnant liver volume was suggested. KGR of liver volume decreased with increasing of the initial remnant liver volume. A positive correlation between KGR of liver volume and LU15 was observed. The relationship between KGR of remnant liver LU15 (/day or %/day) and initial remnant liver volume or LU15 was also plotted for all patients (Fig. 3). Same trend was observed for KGR of remnant liver LU15 and initial remnant liver volume or LU15 as the correlation between KGR of liver volume and initial remnant liver volume or LU15 was seen.
Fig. 2.
Correlation between kinetic liver growth rate (KGR) of liver volume and LU15 or initial remnant liver volume. An inverse correlation between KGR of liver volume and initial remnant liver volume was suggested. KGR of liver volume decreased with increasing of the initial remnant liver volume. A positive correlation between KGR of liver volume and LU15 was observed
Fig. 3.
Correlation between kinetic liver growth rate (KGR) of remnant liver LU15 and LU15 or initial remnant liver volume. An inverse correlation between KGR of remnant liver LU15 and initial remnant liver volume was suggested. KGR of remnant liver LU15 decreased with increasing of the initial remnant liver volume. A positive correlation between KGR and LU15 was observed. Same trend was observed for KGR of remnant liver LU15 and initial remnant liver volume or LU15 as the correlation between KGR of liver volume and initial remnant liver volume or LU15 was seen
Out of all patients, two patients had liver failure. There was no association between KGR and liver failure in this study. There were no patient deaths within 90 days. Liver failure did not correlate with KGR or with KGR of remnant liver LU15.
Discussion
Issues related to the size of the future remnant liver have become increasingly relevant in hepatic surgery as the envelope of resectability with larger and more complex hepatic resections continues to expand [14]. PVE is reliable and it has successfully increased the volume of the future remnant liver [15]. More recently the ALPPS procedure has also led to increases in the future remnant liver but the growth rate of ALPPS (34.8 cm3/day) compared with that of PVE (3 cm3/day) was 11 times greater [10]. In this research, KGR of ALPPS (19.0 cm3/day) was significantly greater than KGR of PVE (3.89 cm3/day), and same trend was observed in the remnant liver LU15 KGR. Importantly, we also showed that both remnant liver volume and function growth in the ALPPS was greater than in the PVE. The perspective presented by both variables of volume and function is novel, as most studies investigating liver hypertrophy report only volumetry of the remnant liver based on CT imaging [16].
We also sought to determine whether KGR was related to initial remnant liver volume and initial remnant liver total LU15. When KGR was plotted against the initial remnant liver volume, the correlation showed a clear linear trend of increasing KGR with a smaller remnant liver volume regardless of the procedure. These findings support the noteworthy observation that future remnant liver volume growth after ALPPS is marked. In fact, based on the volume of the future remnant liver, future remnant liver volume growth after ALPPS reflects a response that is expected after an extended hepatectomy. We have also demonstrated that both the volume and function of future remnant liver growth. Interestingly the correlation between KGR and initial total liver LU15 showed positive linear trend in all patients. In these findings, it was expected that the larger the initial total liver LU 15 and the smaller the initial remnant liver volume, the larger was KGR. Regarding the function, these results might be reasonable, because large LU 15 indicated that the remnant liver function was good.
Some authors have suggested that major liver resections should be avoided in patients with low KGR because these patients have a strongly increased risk of post-operative liver failure [15]. Interestingly others have shown that in selected patients with insufficient future remnant liver growth after PVE salvage the ALPPS approach has provided adequate growth [17]. In this study, the smaller the initial remnant liver volume, the greater was the KGR. No patient was seen with liver failure after PVE procedure. Small-for-size syndrome was only observed after liver resection surgery, especially after living donor liver transplantation. The reason why the situation of small-for-size syndrome was observed after the surgery, and not after PVE was unclear.
In conclusion, we have demonstrated a greater future remnant liver volume and KGR after the ALPPS procedure than after PVE. We have also demonstrated that KGR of patients undergoing the ALPPS procedure appears to be inversely related to the initial remnant liver volume and positively related to the initial total liver LU15. The important point of the present study is that liver hypertrophy is related to the initial remnant liver volume and total liver function.
Conclusion
Future remnant liver volume and KGR was greater after the ALPPS procedure than after PVE. Liver hypertrophy is related to the expected remnant liver volume and total liver function. This study suggested that total liver function and initial remnant liver volume might be a new indication of hepatectomy after PVE and ALPPS in the case of insufficient remnant liver volume.
Availability of data and materials
All authors and all reviewer had availability of these data and materials.
Authors’ contributions
NC and SK participated in the design of the study. NC drafted the manuscript and performed the statistical analysis. SK conceived of the study, and participated in its design and coordination and helped to draft the manuscript. All authors performed operation procedures, read and approved the final manuscript.
Ethics approval and consent to participate
This research data was approved by Tokyo Medical Universoty Hachioji Medical Center Ethics committee.
Consent for publication
This research data had consent of publications from all patents.
Competing interests
The authors declare that they have no competing interests.
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Contributor Information
Naokazu Chiba, Phone: +81-42-665-5611, Email: nchiba0632@yahoo.co.jp.
Kei Yokozuka, Email: myougadani83@gmail.com.
Shigeto Ochiai, Email: kqxcg682@ybb.ne.jp.
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Koichi Tomita, Email: kouichit1983@yahoo.co.jp.
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Shigeyuki Kawachi, Email: skawachi@tokyo-med.ac.jp.
References
- 1.Clavien PA, Petrowsky H, DeOliveira ML, Graf R. Strategies for safer liver surgery and partial liver transplantation. N Engl J Med. 2007;356:1545–1559. doi: 10.1056/NEJMra065156. [DOI] [PubMed] [Google Scholar]
- 2.Arii S, Yamada Y, Futagawa S, Inoue K, Kobayashi K, Kojiro M, et al. Results of surgical and nonsurgical treatment for small-sized hepatocellular carcinomas: a retrospective and nationwide survey in Japan. The liver Cancer study Group of Japan. Hepatol. 2000;32:1224–1229. doi: 10.1053/jhep.2000.20456. [DOI] [PubMed] [Google Scholar]
- 3.Kudo M, Todo A, Ikekubo K, Yamamoto K, Vera DR, Stadalnik RC. Quantitative assessment of hepatocellular function through in vivo radioreceptor imaging with technetium 99m galactosyl human serum albumin. Hepatol. 1993;17:814–819. [PubMed] [Google Scholar]
- 4.Kwon AH, Ha-Kawa SK, Uetsuji S, Kamiyama Y, Tanaka Y. Use of technetium 99m diethylenetriamine-pentaacetic acid–galactosyl-human serum albumin liver scintigraphy in the evaluation of preoperative and postoperative hepatic functional reserve for hepatectomy. Surg. 1995;117:429–434. doi: 10.1016/S0039-6060(05)80063-7. [DOI] [PubMed] [Google Scholar]
- 5.Kokudo N, Vera DR, Koizumi M, Seki M, Sato T, Stadalnik RC, et al. Recovery of hepatic asialoglycoprotein receptors after major hepatic resection. J NuclMed. 1999;40:137–141. [PubMed] [Google Scholar]
- 6.Chiba N, Shimazu M, Takano K, Oshima G, Tomita K, Sano T, et al. Predicting hepatic failure with a new diagnostic technique by preoperative liver scintigraphy and computed tomography: a pilot study in 123 patients undergoing liver resection. Patient Saf Surg. 2017;11:29. doi: 10.1186/s13037-017-0143-z. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7.Abdalla EK, Barnett CC, Doherty D, Curley SA, Vauthey JN. Extended hepatectomy in patients with hepatobiliary malignancies with and without preoperative portal vein embolization. Arch Surg. 2002;137:675–681. doi: 10.1001/archsurg.137.6.675. [DOI] [PubMed] [Google Scholar]
- 8.Liu H, Zhu S. Present status and future perspectives of preoperative portal vein embolization. Am J Surg. 2009;197:686–690. doi: 10.1016/j.amjsurg.2008.04.022. [DOI] [PubMed] [Google Scholar]
- 9.Schnitzbauer AA, Lang SA, Goessmann H, Nadalin S, Baumgart J, Farkas SA, et al. Right portal vein ligation combined with in situ splitting induces rapid left lateral liver lobe hypertrophy enabling 2-staged extended right hepatic resection in small-for-size settings. Ann Surg. 2012;255:405–414. doi: 10.1097/SLA.0b013e31824856f5. [DOI] [PubMed] [Google Scholar]
- 10.Knoefel WT, Gabor I, Rehders A, Alexander A, Krausch M, Schulte am Esch J, et al. In situ liver transection with portal vein ligation for rapid growth of the future liver remnant in two-stage liver resection. Br J Surg. 2013;100:388–394. doi: 10.1002/bjs.8955. [DOI] [PubMed] [Google Scholar]
- 11.Schadde E, Ardiles V, Slankamenac K, Tschuor C, Sergeant G, Amacker N, et al. ALPPS offers a better chance of complete resection in patients with primarily unresectable liver tumors compared with conventional-staged hepatectomies: results of a multicenter analysis. World J Surg. 2014;38:1510–1519. doi: 10.1007/s00268-014-2513-3. [DOI] [PubMed] [Google Scholar]
- 12.Shindoh J, Truty MJ, Aloia TA, Curley SA, Zimmitti G, Huang SY, et al. Kinetic growth rate after portal vein embolization predicts posthepatectomy outcomes: toward zero liver-related mortality in patients with colorectal liver metastases and small future liver remnant. J Am Coll Surg. 2013;216(2):201–209. doi: 10.1016/j.jamcollsurg.2012.10.018. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 13.Rahbari NN, Garden OJ, Padbury R. Posthepatectomy liver failure: a definition and grading by the international study Group of Liver Surgery (ISGLS) Surg. 2011;145:713–724. doi: 10.1016/j.surg.2010.10.001. [DOI] [PubMed] [Google Scholar]
- 14.Croome KP, Hernandez-Alejandro R, Parker M, Heimbach J, Rosen C, Nagorney DM. Is the liver kinetic growth rate in ALPPS unprecedented when compared with PVE and living donor liver transplant? A multicentre analysis. HPB (Oxford) 2015;17(6):477–484. doi: 10.1111/hpb.12386. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 15.Vauthey JN, Abdalla EK, Doherty DA, Gertsch P, Fenstermacher MJ, Loyer EM, et al. Body surface area and body weight predict total liver volume in western adults. Liver Transpl. 2002;8:233–240. doi: 10.1053/jlts.2002.31654. [DOI] [PubMed] [Google Scholar]
- 16.Correa D, Schwartz L, Jarnagin WR, Tuorto S, DeMatteo R, D’Angelica MI, et al. Kinetics of liver volume changes in the first year after portal vein embolization. Arch Surg. 2010;145:351–354. doi: 10.1001/archsurg.2010.42. [DOI] [PubMed] [Google Scholar]
- 17.Tschuor C, Croome KP, Sergeant G, Cano V, Schadde E, Ardiles V, et al. Salvage parenchymal liver transection for patients with insufficient volume increase after portal vein occlusion – an extension of the ALPPS approach. Eur J Surg Oncol. 2013;39:1230–1235. doi: 10.1016/j.ejso.2013.08.009. [DOI] [PubMed] [Google Scholar]