FIG. 1.

Determination of pharmacological injury doses—1 nL injection at 3 dpf. (A) Representative images showing pericardial edema (arrows) and remaining yolk reserves (*) presented in a portion of larvae administered adriamycin or puromycin. (B) Five milligrams per milliliter puromycin does not result in edema. Increasing the puromycin dose to 25 mg mL⁻¹ causes edema in >40% of fish from 24 hpi (mean ± SEM). (C) Four milligrams per milliliter adriamycin causes edema in >75% of larvae by 24 hpi (mean ± SEM). SEM, standard error of the mean; dpf, days postfertilization; hpi, hours postinjection.