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. 2018 Jun 4;7:e33286. doi: 10.7554/eLife.33286

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© 2018, Zhou et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 3. — (A) LC-MS traces of the reaction of ACS-7, ACS-7(E339A) single mutant, or ACS-7(E339A, S186A, S187A) triple mutant with IC-asc-C9, detecting IC-asc-C9-CoA. (B–C) LC-MS traces of the reaction of ACS-7, ACS-7 (E339A), or ACS-7 (E339A, S186A, S187A) with ICA, detecting IC-AMP (B) or IC-CoA (C). (D) Activity of ACS-7 in a coupled enzyme assay against a panel of substrates, including fatty acids (fatty acid-C7 and fatty acid-C9), ascarosides (asc-C7, asc-ΔC9, and asc-C9), IC-modified ascarosides (IC-asc-C5, IC-asc-C7, IC-asc-ΔC9, and IC-asc-C9), and ICA. Data represent the mean ± SD of three independent experiments.