Figure 4.

GEM alters the immunopeptidome of tumour cells. a) Relative abundance of peptides from MDM2, RPA70 and TOPO1 proteins was assessed in control and GEM-treated HCT116 cells. n = 2. b) The relative abundance of all peptides present in both control and GEM treated cells in all three experiments is shown. Values significantly different from controls by student's paired t-test are indicated (* = p<0.05). c) Western blots showing the expression of proteins that have an altered peptide representation in GEM-treated cells are shown. Blots are representative of three separate experiments.