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. 2018 Jun 5;26(6):879–886.e3. doi: 10.1016/j.str.2018.03.015

Figure 2.

Figure 2

Cryo-ET of Preselected Areas and Fiducial-Based Correlation to Identify Eisosomes by Pil1-GFP Localization

(A) A single focal plane in cryo-FM, merge of green and red (shown in magenta) channels. White circle indicates a Pil1-GFP signal selected for cryo-ET.

(B) Intermediate-magnification EM image. White dashed rectangle indicates the field of view imaged by cryo-ET (C). Yellow circles in (A and B) indicate fiducial markers for high-precision localization of the Pil1-GFP signal (center of green dashed circle).

(C) Virtual slice through the tomographic volume.

(D) Magnified view of the dashed rectangle shown in (C). Arrows in (C and D) indicate a furrow-like invagination.

Scale bars represent 2 μm in (A and B), 100 nm in (C), and 25 nm in (D). See also Figure S2.