REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
TetraSpeck Microspheres 100 nm	Invitrogen / Thermo Fisher Scientific	Catalog number T7279
TetraSpeck Microspheres 50 nm	Invitrogen / Thermo Fisher Scientific	Custom order
Dextran from Leuconostoc spp. Mr ∼40000	Sigma	Product 68084
Lowicryl HM20 embedding kit	Polysciences, Inc.	Catalog number 15924
Deposited Data
Electron cryo-tomogram of vitreous section of yeast cell	This paper, deposited at EMDB	EMD-4305
Electron cryo-tomographic tilt series and corresponding correlative microscopy data	This paper, deposited at EMPIAR	EMPIAR-10161
Experimental Models: Organisms/Strains
S. cerevisiae strain: MATα, his3Δ200, leu2-3,112, ura3-52, lys2-801, PIL1-EGFP::HIS3MX6	This paper	WKY0207
Software and Algorithms
RELION	Bharat et al., 2015 Scheres, 2012	http://www2.mrc-lmb.cam.ac.uk/relion
MATLAB-based correlation scripts	Kukulski et al., 2011 Schorb et al., 2016	NA
SerialEM	Mastronarde 2005	http://bio3d.colorado.edu/SerialEM/
IMOD	Kremer et al., 1996	http://bio3d.colorado.edu/imod/
CTFFIND	Mindell and Grigorieff, 2003	NA
Other
QUANTIFOIL EM grids (copper, 200 mesh, R3.5/1)	www.quantifoil.com	No product code
Gold-coated copper specimen carrier type A	Wohlwend GmbH, Sennwald, Switzerland	Art. 662
Aluminium specimen carrier type B	Wohlwend GmbH, Sennwald, Switzerland	Art. 242
Aluminium specimen carrier type A	Wohlwend GmbH, Sennwald, Switzerland	Art. 241
Carbon film EM grids (copper, 200 mesh)	Agar Scientific	Code AGS160