Table 1.

DNA oligonucelotides used for primers of the RT-PCR experiments

Oligoa	Sequence (5’-3’)	Location in the gene	Gene	PCR products
FL594F	ATTATGGCCCACACCAGTGGCGC	2917–2939	LacZ	173bp, distalb
FL594R	TGACGGGCTCCAGGAGTCGTC	3069–3089	LacZ	173bp, distalb
FL654F	CACCGATCGCCCTTCCCAACAGTTG	212–236	LacZ	172bp, proximalc
FL654R	GTAGATGGGCGCATCGTAACCG	362–383	LacZ	172bp, proximalc
FL580F	GCGAGGCTGGATGGCCTTCC	990–1009	tet	176bp, distalb
FL580R	CCGTGACGATCAGCGGTCCAG	1145–1165	tet	176bp, distalb
FL657F	GGCCTCTTGCGGGATATCGTCCATTCC	184–210	tet	168bp, proximalc
FL657R	GATAGTGGCTCCAAGTAGCGAAGCG	327–351	tet	168bp, proximalc
FL590F	TCCAATTGGCGATGGCCCTGT	660–680	GFPuv	101bp
FL590R	GGACCATGTGGTCACGCTTTTCGT	737–760	GFPuv	101bp
FL586F	AGTTATCCCCCTCCATCAGG	154–135	16S rRNA	99bp
FL586R	TGCAAGTCGAACGGTAACAG	56–75	16S rRNA	99bp

^aFLXXXF and FLXXXR represent the forward and reverse primers of the PCR reactions, respectively.

^bDistal indicates the PCR products that locate in the distal region of the gene.

^cProximal indicates the PCR products locating in the proximal region of the gene.