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. 2017 Dec 22;28(2):69–79. doi: 10.1093/glycob/cwx095

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© The Author(s) 2017. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 6. — Specificity of T and Tn antigens imaging. (A) Schematic view of the synthesis of T and Tn antigens and their detection. The antigens are installed to proteins using GALNT2 and C1GalT1 in the presence of donor substrates UDP-GalNAc and UDP-Gal. T antigen is detected by GCNT1. Tn antigen is detected by B3GNT6. (B) Fully installed T antigen (core-1 O-glycan) on HUVEC cells imaged by GCNT1. (C) In the absence of UDP-Gal, only Tn antigen was synthesized, which resulted in negative cell staining by GCNT1. (D) In the absence of UDP-Gal, only Tn antigen was synthesized, which resulted in positive cell staining by B3GNT6. (E) Fixed HUVEC cells were directly stained by B3GNT6. Staining in B and C indicated by the yellow arrows may due to mis-folded and incompletely glycosylated protein aggregates. Antigen staining was revealed by Alexa-Fluor 555 (red) and nuclei were revealed by DAPI (blue).