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. 2018 May 4;20(6):563–573. doi: 10.1016/j.neo.2018.04.003

Figure 5.

Figure 5

Both mTOR and eIF4E phosphorylation are needed for polysomal recruitment of terminal oligopyrimidine messenger RNAs (TOP mRNA). Inhibition of mTOR by rapamycin prevents polysomal recruitment of TOP mRNA. However, rapamycin also induced the phosphorylation of eIF4E at S209, which was also needed for TOP mRNA recruitment. This shows that inhibition of the PI3K/Akt/mTOR pathway moved signal transduction towards a Ras/MAPK pathway, resulting in an increase in eIF4E phosphorylation. Therefore, both rapamycin and an Mnk inhibitor were needed to completely inhibit protein translation.