Characteristics of Synchronous and Metachronous Multiple Gastric Tumors after Endoscopic Submucosal Dissection of Early Gastric Neoplasm

Abstract

Background/Aims

Endoscopic submucosal dissection (ESD) has been widely accepted as a method of treatment of early gastric tumor. This study aimed to identify the incidence and characteristics of multiple gastric tumors after ESD.

Methods

Patients with early gastric tumors who were treated by ESD from January 2004 to June 2012 and followed up with endoscopic examination periodically for at least 1 year were enrolled. All multiple gastric lesions were subsequently treated with ESD and the medical records of the patients were retrospectively reviewed.

Results

In total, 643 patients were included. The mean duration of endoscopic follow-up was 45.27±27.59 (range, 12–148) months. Overall, 144 patients (22.4%) showed multiple gastric tumors during the follow-up period (44 synchronous [6.8%] and 100 metachronous [15.5%]). The cumulative incidence rate steadily increased during the follow-up period. More than 50% of the tumors that developed at the same longitudinal location of the stomach were of the same macroscopic and histological type as the primary lesions.

Conclusions

Because synchronous and/or metachronous gastric tumors are common, considerable attention should be paid to detect multiple gastric lesions after ESD of early gastric neoplasm.

INTRODUCTION

Early gastric cancer (EGC) is defined as a neoplasm confined to the mucosa or submucosa regardless of the presence of regional lymph node metastasis [1,2]. Gastric dysplasia is defined as benign unequivocal neoplastic epithelial lesion without histologic evidence of invasive carcinoma and reparative changes [3]. Currently, the diagnostic rate of EGC and gastric dysplasia has increased owing to improved diagnostic procedures and nationwide surveillance programs. The incidence of gastric dysplasia has been reported between 9% and 20% in high-risk areas for gastric cancers [4].

Endoscopic resection has been widely accepted as the standard treatment for EGC without the risk of regional lymph node metastasis, because it has the advantages of being less invasive, and comparable with surgery [5-7]. Many studies have demonstrated that gastric dysplasia can also be treated by endoscopic resection because it has a risk of malignancy and should be regarded as a precursor of gastric cancer [8]. Among several methods of endoscopic resection, endoscopic submucosal dissection (ESD) has advantages over endoscopic mucosal resection for treating early gastric neoplasm consisting of EGC and gastric dysplasia, in that it allows en bloc resection and has an expanding range of indications [9-11].

Consequently, the number of medical facilities that perform gastric tumor ESD has increased, and multiple gastric tumors after ESD have become an emerging concern owing to the widespread acceptance of ESD. However, the majority of published literature to date has reported on synchronous lesions after gastrectomy or endoscopic resection of EGC [12-14]. Data on metachronous lesions after ESD for early gastric neoplasms are still limited, and the long-term results of the incidence of metachronous tumor after ESD have not been fully evaluated. Therefore, we performed a retrospective analysis of the incidence and characteristics of synchronous and metachronous multiple gastric tumors after ESD of early gastric neoplasm over a long-term follow-up.

MATERIALS AND METHODS

Patients

ESD was performed in 1,390 patients at Keimyung University Dongsan Hospital in Daegu, Korea, for the treatment of early gastric neoplasm between June 2004 and June 2012. All patients were recommended to undergo endoscopy at 3, 6, and 12 months after the procedure, and yearly follow-up thereafter. If patients had multiple lesions at the time of ESD, only the main lesions were included in the analysis. The main lesion was defined as the most malignant or dysplastic lesion [12]. If multiple lesions had the same histology, the largest lesion was regarded as the main lesion. Longitudinal tumor locations were classified as being in the upper (cardia, fundus, and upper body), middle (mid body, lower body, and angle), and lower (antrum and prepylorus) parts of the stomach [15]. Circumferential tumor locations were defined as anterior wall, posterior wall, lesser curvature, and greater curvature [12]. The maximum tumor diameter in the gastric neoplasm was macroscopically estimated. The endoscopic gross morphology of the tumors was classified based on the Paris classification [16]. The presence of atrophy was reviewed endoscopically by an expert endoscopist, and intestinal metaplasia was histologically assessed based on the resected specimen. The study was approved by the ethics committee (Review board of Keimyung University Dongsan Hospital (No. 13-272). Written informed consent was obtained from all patients.

Definitions

We classified multiple gastric tumors based on the time at which the lesions were detected [17]. Multiple gastric tumors detected within 1 year of initial ESD were classified as synchronous lesion. Metachronous lesion was defined as a new gastric tumor in the area other than the site of primary lesion and diagnosed after 1 year of the initial ESD. We simply reclassified endoscopic gross types of gastric tumors into three groups: polypoid (type 0–I), flat elevated (type 0–IIa, 0–IIb, and 0–IIa+IIc), and superficial depressed (type 0–IIc, 0–III, and 0–IIc+IIa). Helicobacter pylori status was considered to be positive if the result of histology, rapid urease test, or urea breath test was positive.

Endoscopic submucosal dissection methods

Standardized ESD procedure was performed in patients under conscious sedation by three experts, each of whom had performed ESD more than 100 times for more than 2 years before the procedure. Several spots were marked with needle knife or argon plasma coagulation 5 to 10 mm outside the margin of the lesion. Subsequently, hypertonic saline mixed with epinephrine (1:10,000) was injected into the submucosa to lift the lesion. The IT knife or hook knife was inserted into the initial incision, and electrosurgical current was applied using an electrosurgical generator. All patients fasted after the procedure along with the use of a proton pump inhibitor. Endoscopy was performed within 3 days to confirm the periprocedural bleeding.

Evaluation of clinicopathologic feature

The resected specimens were stretched, pinned to a polystyrene plate, and embedded in 8% formaldehyde for 24 h for fixation. The fixed specimen was sectioned before histological processing, and staining with hematoxylin and eosin. All resected specimens were evaluated by histopathologic examination based on the Vienna classification [18]. The final pathologic diagnoses were classified as dysplasia and gastric cancer with differentiated or undifferentiated type, according to the Japanese classification [19].

Statistical analysis

Data were analyzed using the Fisher’s exact or Chi-square tests. To compare continuous variables among groups, ANOVA test was performed, as appropriate. The follow-up period was calculated from the date of the initial ESD to the detection date of the first synchronous and metachronous multiple gastric tumors or the date of the last endoscopic examination for patients in whom multiple lesions were not diagnosed. The cumulative incidence rate of synchronous or metachronous tumors was plotted using the Kaplan-Meier method. All data analyses were performed using SPSS version 22.0 (SPSS Inc., Chicago, IL, USA). P-values <0.05 were considered statistically significant.

RESULTS

Baseline patient and lesion characteristics

We retrospectively reviewed the prospectively collected ESD database system. Endoscopic resection was performed in 1,390 patients, and we excluded 362 patients whose histopathologic result was piecemeal, margin positive, or undetermined. We excluded 125 patients who underwent subsequent surgical resection owing to non-curative resection, 234 patients who were lost to follow-up within 1 year, and 11 patients who underwent prior gastrectomy before ESD. Fifteen patients who had carcinoid tumor after ESD also dropped out of the study. Finally, 643 patients (499 with a solitary lesion and 144 with two or more synchronous or metachronous lesions; 355 EGC and 288 adenomas) who underwent ESD for early gastric neoplasm met the inclusion criteria for this study (Fig. 1).

Fig. 1.

Flow chart of included patients. ESD, endoscopic submucosal dissection. ^a)Piecemeal, margin-positive, or undetermined.

Incidences and characteristics of synchronous and metachronous lesion

Consecutive 643 patients were included in this study. The mean period of endoscopy follow-up was 45.27±27.59 (range, 12–148) months. Overall, 144 patients (22.4%) showed multiple gastric tumors during the follow-up period. Among them, 44 (6.8%) synchronous lesions were detected within 1 year of the initial ESD. Thirteen synchronous lesions were already found at the time of endoscopic resection, and 31 were detected within 1 year of endoscopic resection. Of these patients, 22 subsequently developed metachronous tumors during the follow-up period, and they were classified as the metachronous lesion subgroup in our study. Finally, metachronous lesions had developed in 100 (15.5%) patients. Of 144 multiple gastric tumors, 43 (29.9%) were differentiated type-cancers, 8 (5.6%) were undifferentiated-type cancers, and 93 (64.6%) were adenomas. Of these patients, nine subsequently developed third multiple gastric tumors.

Cumulative incidences of synchronous and metachronous lesion

The cumulative incidence curve of multiple synchronous and metachronous tumors showed a linear increase. The cumulative incidence rates at 3, 5, 7, and 9 years were 18.5%, 29.6%, 38.7%, and 47.5%, respectively (Fig. 2).

Fig. 2.

Cumulative incidence curve of synchronous and metachronous gastric tumor after endoscopic submucosal dissection of early gastric neoplasm.

Fig. 3 shows the Kaplan-Meier plots for multiple gastric tumors based on the histologic differences of the primary lesion. The cumulative incidence showed no significant difference between the dysplasia and cancer groups (p=0.525). The cumulative incidence curve of synchronous and metachronous tumors between patients older than 60 years and those younger than 60 years is plotted in Fig. 4 (p=0.072).

Fig. 3.

Kaplan-Meier analysis of the incidence rate of multiple gastric tumors in patients according to histologic differentiation of the primary lesion (p=0.525).

Fig. 4.

Kaplan-Meier analysis of the incidence rate of multiple gastric tumors in patients by age at diagnosis (p=0.072).

The mean time interval from the initial ESD procedure to the diagnosis of secondary multiple neoplasms was approximately 5.1 (range, 1–10.3) months and 38.2 (range, 12–124.6) months in synchronous and metachronous tumors, respectively.

Comparison of clinicopathologic characteristics of the patients and initial lesions between solitary and multiple lesion groups

Table 1 shows the patient and lesion characteristics with early gastric neoplasm and their initial lesions. The male-to-female patient ratio was approximately 2:1 (428 men and 215 women). The mean age at diagnosis of the synchronous lesion was the highest among the three groups, and it decreased stepwise in the order of metachronous and solitary groups (69.6±8.6, 66.0±8.5 and 65.3±9.1 years, respectively, p=0.009). The mean lesion size of the synchronous group was 16.9±12.2 mm and larger than the lesion size in other groups; however, the difference in lesion size among the groups was not statistically significant. The characteristics of the initially resected lesions, including location, number, gross type, histological type, and invasion depth had no significant association with the development of secondary multiple tumors. In addition, no significant differences were found in patient comorbidities among the three groups.

Table 1.

Characteristics of Patients with Gastric Mucosal Neoplasm and Their Initial Lesions

Factors (n)	Solitary (n=499)	Synchronousa) (n=44)	Metachronous (n=100)	p-value
Age at diagnosis, mean±SD	65.3±9.1	69.6±8.6	66.0±8.5	0.009
Sex (M:F)	1:0.5	1:0.2	1:0.5	0.108
Location (long axis)				0.676
Upper	28 (5.6%)	3 (6.8%)	5 (5.6%)
Middle	154 (30.9%)	18 (40.9%)	33 (33.0%)
Lower	317 (63.5%)	23 (52.3%)	62 (62.0%)
Location (short axis)				0.660
Anterior wall	116 (23.2%)	7 (15.9%)	21 (21.0%)
Lesser curvature	188 (37.7%)	15 (34.1%)	34 (34.0%)
Posterior wall	106 (21.2%)	12 (27.3%)	21 (21.0%)
Greater curvature	89 (17.8%)	10 (22.7%)	24 (24.0%)
Tumor size (mm, mean±SD)	14.8±11.1	16.9±12.2	14.8±11.8	0.250
Tumor size ≥20 mm	125 (25.1%)	11 (25.0%)	23 (23.0%)	0.891
Gross type				0.105
Polypoid	34 (6.8%)	4 (9.1%)	2 (2.0%)
Flat elevated	360 (72.1%)	36 (81.8%)	79 (79.0%)
Depressed or ulcer	105 (21.0%)	4 (9.1%)	19 (19.0%)
Histology				0.557
Differentiated cancer	267 (50.5%)	28 (60.9%)	36 (45.0%)
Undifferentiated cancer	26 (4.9%)	2 (4.3%)	4 (5.0%)
Dysplasia	236 (44.6%)	16 (34.8%)	40 (50.0%)
Multiple lesions at initial ESD	97 (19.4%)	13 (29.5%)	21 (21.0%)	0.276
Depth of invasion				0.348
Mucosa	468 (93.8%)	39 (88.6%)	95 (95.0%)
Submucosa	31 (6.2%)	5 (11.4%)	5 (5.0%)
Comorbidities
Hypertension	183 (36.7%)	19 (43.2%)	29 (29.0%)	0.203
Diabetes mellitus	83 (16.6%)	5 (11.4%)	15 (15.0%)	0.620
Cardiovascular disease	27 (5.4%)	4 (9.1%)	10 (10.0%)	0.181
Cerebrovascular disease	25 (5.0%)	3 (6.8%)	2 (2.0%)	0.361
Liver cirrhosis	7 (1.4%)	1 (2.3%)	1 (1.4%)	0.668
Chronic kidney disease	10 (2.0%)	0	1 (1.0%)	0.864

SD, standard deviation; ESD, endoscopic submucosal dissection.

^a)Twenty two patients had both synchronous and metachronous lesions. In this analysis, they are grouped with the metachronous lesion subgroup.

Clinicopathological similarities of multiple gastric tumors

The clinicopathologic features of the 144 lesions with synchronous or metachronous gastric tumor are shown in Table 2. More than 50% of the multiple tumors were revealed to have the same gross type as the primary lesion had and to be located in the same longitudinal location of the stomach (67.4% and 54.2%, respectively). Irrespective of the macroscopic type of the primary tumor, the most common type of multiple gastric tumors is the flat elevated type (72.9%). More than 50% of multiple gastric tumors were discovered to be of the same histologic type as the primary tumor (54.2%).

Table 2.

Clinicopathological Similarities of Multiple Gastric Tumors

Primary → secondary	Synchronous (44 lesions)	Metachronous (100 lesions)	Total (144 lesions)
Gross type
Polypoid → polypoid/flat elevated/depressed	0/2/2	0/2/0	0/4/2
Flat elevated → polypoid/flat elevated/depressed	4/28/4	7/60/12	11/88/16
Depressed → polypoid/flat elevated/depressed	0/4/0	1/9/9	1/13/9
Same gross type	28 (63.6%)	69 (69.0%)	97 (67.4%)
Location (long axis)
Upper → upper/mid/lower	1/1/1	1/3/1	2/4/2
Mid → upper/mid/lower	4/8/6	3/15/15	7/23/21
Lower → upper/mid/lower	2/8/13	3/19/40	5/27/53
Same location	22 (50.0%)	56 (56.0)	78(54.2%)
Location (short axis)
AW → AW/LC/PW/GC	0/1/6/0	5/6/5/5	5/7/11/5
LC → AW/LC/PW/GC	5/6/1/3	8/12/10/4	13/18/11/7
PW → AW/LC/PW/GC	2/5/4/1	3/8/5/5	5/13/9/6
GC → AW/LC/PW/GC	5/1/2/2	3/3/7/11	8/4/9/13
Same location	12 (27.3%)	33 (33.0%)	45 (31.3%)
Histologic type
Cancer → cancer/dysplasia	10/18	23/30	33/48
Dysplasia → cancer/dysplasia	4/12	14/33	18/45
Same histologic type	22 (50.0%)	56 (56.0%)	78 (54.2%)

AW, anterior wall; LC, lesser curvature; PW, posterior wall; GC, greater curvature.

DISCUSSION

ESD is accepted as a standard treatment for early gastric neoplasm; but the incidence of metachronous tumor after ESD was higher than that after gastrectomy, because ESD preserves most of the stomach [20,21]. The incidence of metachronous cancer after endoscopic resection has been reported to range from 5.1% to 14% [6,22-24]. In this study, patients with metachronous gastric tumor accounted for 15.5% of the total patients, which was similar to previous reports, but was relatively high. The cumulative incidence of multiple gastric tumors increased constantly after ESD in our study. This result can be extrapolated by the large proportion of patients with dysplasia (44.8%) in our study. We speculated that gastric dysplasia should be included because it is considered to be a direct precursor of gastric adenocarcinoma, and a recently reported guideline has also recommended complete removal of gastric dysplasia when it is safe to do so [25]. This difference may be caused by the strict inclusion criteria in the present study. We included subjects histologically confirmed to have undergone complete curative resection and excluded those who had undergone subsequent surgical resection. As a result, very small proportion of patients with undifferentiated or submucosal invasive cancer was included in our study (Table 1). We attempted to distinguish synchronous or metachronous lesions from local recurrence and increased the reliability of our results.

Among the 643 patients analyzed in this study, the rate of synchronous multiple lesions was 6.8% (44/643), which was slightly lower than that reported in other studies (9%–14%) [12,26-28]. This may be related to 22 patients who had both synchronous and metachronous lesions that were grouped with the metachronous lesion subgroup in our study. As a result, the real incidence of synchronous lesions after ESD may be higher than the 6.8% calculated in the present study. Therefore, it would be more accurate to state that the lowest incidence of synchronous neoplasm is 7.0%.

The cumulative incidence rate steadily increased during the follow-up period. At 5 years after ESD, multiple gastric tumors developed in approximately 30% of ESD cases in early gastric neoplasm. These findings imply that synchronous and metachronous lesions are not rare, because the recent progress in endoscopic examination has resulted in increased detection of multiple gastric tumors. In clinical practice, the follow-up frequency of each patient varies widely, because frequent endoscopic follow-up limits the quality of life of the patients and increases the overall medical cost [6]. The appropriate time limit for surveillance of multiple gastric tumors is unclear, because only few studies on metachronous tumors were assessed more than 10 years after endoscopic resection [6,29]. Scheduled endoscopic surveillance is necessary and might help detect multiple gastric tumors, but no standardized consensus for endoscopic surveillance period and interval for secondary gastric tumors after ESD exists [30,31]. Therefore, identification of high-risk patients is important for determining a schedule of endoscopic surveillance after ESD. Previous studies suggested that old age is a significant risk factor for multiple gastric neoplasms [22,32]. Similarly, the cumulative incidence was higher in elderly patients aged more than 60 years in the present study (p=0.072). The reason for old age being associated with metachronous tumor is unclear; it may be attributed to the multistep model of gastric carcinogenesis. Cahill et al. suggested a trend toward increased cell replication from normal mucosa to atrophic gastritis, to intestinal metaplasia to carcinoma [33]. In older individuals, the gastric glands are generally atrophic with the increase of intestinal metaplasia. Thus, the majority of gastric cancers that develop in elderly patients are a background of intestinal metaplasia, and these cancers are frequently associated with subsequent multiple gastric lesions [34].

H. pylori infection has been regarded as one of the important causes of gastric cancer [35]. In this study, data for H. pylori infection status was missing in 210 patients and 433 patients with any of the H. pylori tests results were included. H. pylori infection was identified in 189 (56.6%), 16 (53.3%) and 41 (59.4%) patients in the solitary, synchronous and metachronous groups, respectively. Among 246 patients with H. pylori-positive results, 138 patients (56.1%) underwent eradication therapy. The metachronous gastric neoplasms occurred more frequently in H. pylori-infected patients, which is similar to other reports [36,37], however no significant difference (p=0.750) was found between the groups. The period of H. pylori infection is possibly presumed to be closely related with the occurrence of atrophic gastritis, intestinal metaplasia, and subsequent gastric neoplasms. Considering the multistep processes that started from chronic inflammation of the gastric mucosa, which slowly progresses to atrophic gastritis, intestinal metaplasia, and dysplasia to gastric adenocarcinomas [38], H. pylori uninfected status may inhibit the growth rate of metachronous gastric neoplasms [35]. A recently reported prospective, randomized study also suggested that H. pylori eradication might delay the growth of metachronous cancer [39]. Although several studies have reported that H. pylori eradication can reduce the multifocal development of metachronous lesions, the effect of eradication after endoscopic resection for gastric neoplasms remains debatable [37,39-41]. In our study, H. pylori infection status were small in number and few patients underwent urea breath test or histologic evaluation to confirm successful eradication; thus, we cannot analyze the effect of eradication of H. pylori on the development of secondary multiple neoplasms after ESD. Larger studies are required to clarify this issue.

More than 50% of the neoplasms in the 144 patients with multiple gastric lesions during the follow-up period developed at the same longitudinal location of the stomach, and showed the same gross type and histopathological type as that shown by the primary lesions (54.2%, 67.4%, and 54.2%, respectively). A few studies have suggested that the histologic type of tumor and tumor number are important risk factors for multiple gastric neoplasms [12,36]. In our study, these factors had no significant relationship with metachronous occurrence, and the cumulative incidence of multiple gastric tumors based on primary histologic difference did not show a significant difference (Fig. 3). In addition, there was no significant difference in the incidence of synchronous gastric cancer and dysplasia according to the histologic type of the primary tumor (2.8%, 5.1% in primary cancer group vs. 1.4%, 4.2% in primary dysplasia group, p=0.356), and metachronous gastric cancer and dysplasia (6.5%, 8.5% in primary cancer group vs. 4.9%, 11.5% in primary dysplasia group, p=0.372). Most multiple secondary tumors were detected as superficial lesions that imply that these lesions can be removed by subsequent ESD without surgical resection (n=108, 75%). Therefore, the endoscopist should keep these characteristics in mind, and conduct careful follow-ups to facilitate early detection of multiple gastric lesions regardless of the primary histology of the lesions.

Our study had several limitations. First, potential information biases might have occurred owing to the retrospective design of this study. Second, we did not evaluate the severity and extension of intestinal metaplasia and atrophic gastritis, because the grading system of atrophic gastritis and intestinal metaplasia is not well defined owing to their lack of standardization. Third, the data regarding H. pylori infection status were small in number, and the duration of H. pylori infection was uncertain.

In conclusion, as synchronous and/or metachronous gastric tumors are common after ESD of early gastric neoplasm, substantial attention should be paid to detect multiple gastric tumors. The early detection and treatment of multiple gastric tumors after ESD require strict long-term post-procedural surveillance, particularly in elderly patients. Prospective studies on risk factors for multiple gastric tumors and adequate surveillance schedule after ESD of gastric tumors are warranted.