Fig. 1.

Recombinant glycoconjugate vaccine preparations produced in E. coli. 0.5 µg protein separated on SDS-PAGE gel for batch 1 (A/B) and batch 2 (C/D), A/C – Immunoblot with anti-His tag antibody (red) and anti- SP4 capsule antibody (green). SP4 antiserum cross reacts with AcrA. B/D – Coomassie stained gel. A/B. Lane 1: AcrA conjugated to recombinant SP4 polysaccharide. C/D Lane 1: AcrA only; lane 2: AcrA conjugated to C. jejuni heptasaccharide; lane 3: AcrA conjugated to recombinant SP4 polysaccharide. M: molecular weight marker PageRuler Plus. i = unglycosylated AcrA, ii = AcrA glycosylated with single glycan unit at one glycosylation site, iii = AcrA glycosylated with single glycan unit at both glycosylation sites, iv = AcrA glycosylated with polymerized SP4 at one glycosylation site, v = AcrA glycosylated with polymerized SP4 at both glycosylation sites. Images have been cropped for clarity and ease of labelling. Each sub figure contains lanes cropped from the same gel. Separate, uncropped figures for each of the fluorescence channels are presented in supplementary data (Fig. S2 and S3). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)