Fig. 4.

Antibody responces in vaccinated mice. A shows the IgG antibody responces to the carrier protein AcrA in vaccinated mice, measured using a luminex assay. B shows the IgG antibody responces to type 4 polysaccharide in vaccinated mice, measured using a Luminex assay. Antibody responces were measured in sera taken pre challenge with TIGR4. Each dot represent antibody levels in a single animal. Red circular symbol indicates the antibody levels in the mouse that was vaccinated with AcrA-SP4 P2 and survived challenge. Statistical analysis was performed in GraphPad Prism using a Kruskal-Wallis one way ANOVA with a Dunn’s multiple comparison test, *P < 0.05, **P < 0.01, ***P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)