Decadal stability in genetic variation and structure in the intertidal seaweed Fucus serratus (Heterokontophyta: Fucaceae)

Abstract

Background

The spatial distribution of genetic diversity and structure has important implications for conservation as it reveals a species’ strong and weak points with regard to stability and evolutionary capacity. Temporal genetic stability is rarely tested in marine species other than commercially important fishes, but is crucial for the utility of temporal snapshots in conservation management. High and stable diversity can help to mitigate the predicted northward range shift of seaweeds under the impact of climate change. Given the key ecological role of fucoid seaweeds along rocky shores, the positive effect of genetic diversity may reach beyond the species level to stabilize the entire intertidal ecosystem along the temperate North Atlantic. In this study, we estimated the effective population size, as well as temporal changes in genetic structure and diversity of the seaweed F. serratus using 22 microsatellite markers. Samples were taken across latitudes and a range of temperature regimes at seven locations with decadal sampling (2000 and 2010).

Results

Across latitudes, genetic structure and diversity remained stable over 5–10 generations. Stable small-scale structure enhanced regional diversity throughout the species’ range. In accordance with its biogeographic history, effective population size and diversity peaked in the species’ mid-range in Brittany (France), and declined towards its leading and trailing edge to the north and south. At the species’ southern edge, multi-locus-heterozygosity displayed a strong decline from 1999 to 2010.

Conclusion

Temporally stable genetic structure over small spatial scales is a potential driver for local adaptation and species radiation in the genus Fucus. Survival and adaptation of the low-diversity leading edge of F. serratus may be enhanced by regional gene flow and ‘surfing’ of favorable mutations or impaired by the accumulation of deleterious mutations. Our results have clear implications for the conservation of F. serratus at its genetically unique southern edge in Northwest Iberia, where increasing temperatures are likely the major cause for the decline not only of F. serratus, but also other intertidal and subtidal macroalgae. We expect that F. serratus will disappear from Northwest Iberia by 2100 if genetic rescue is not induced by the influx of genetic variation from Brittany.

Electronic supplementary material

The online version of this article (10.1186/s12862-018-1213-2) contains supplementary material, which is available to authorized users.

Background

Understanding temporal stability of genetic structure and diversity is crucial for the utility of temporal snapshots in conservation management and to infer how climate-induced range shifts might affect the future distribution and adaptive potential of species. In trailing edge populations, effective population size and genetic diversity are considered major keys to adaptive potential and subsequent persistence under climate change [1, 2]. In contrast, the evolutionary potential and survival of low-diversity leading edge populations [3] may be either enhanced or impaired by the ‘surfing’ of new mutations that can rapidly increase in frequency over iterated founder events, depending on whether the new mutations are primarily favorable or deleterious [4–9].

Studies that assess temporal genetic stability are rare in marine species, and mostly confined to fisheries management to ensure sustainable exploitation of economically important species [10–14]. While high gene flow explained 5 to 24-year long stability in genetic variability and structure in Chinook salmon and Atlantic herring [10, 11], large fluctuations in allele frequencies were recorded over a few months in small and closed populations of the intertidal isopod Jaera albifrons [15]. However, high gene flow does not always warrant temporal genetic stability, as several marine species with long-lived planktonic larvae showed stronger temporal than spatial differentiation over 3 to 9 years [16–18]. On the other hand, low gene flow does not necessarily result in genetic instability over time, although genetic drift in small and closed populations can be expected to be high. For example, genetic diversity and population structure remained stable over 5–12 years in relatively closed populations of the seagrass Zostera marina [19] and over 2 years in nine out of 10 locally differentiated populations of the isopod Excirolana braziliensis [20]. These contrasting results demonstrate that a species’ life history alone does not necessarily predict its genetic stability over time.

Due to their high sensitivity to rising temperatures, responses of marine intertidal species are considered as early warning signals for the impact of climate change [21–25]. Among global climate change factors, ocean warming is considered the most severe threat for marine macrophytes [26–28]. Over the next century, ecological niche models predict the disappearance of intertidal fucoid brown algae along their southern trailing edges and a poleward extension of their northern leading edges [26, 29]. Fucoid brown algae (Heterokontophyta; Fucaceae) are habitat-forming ecosystem engineers supporting species-rich intertidal communities along temperate rocky shores [30–33]. Thus, range shifts of fucoids will undoubtedly trigger major ecological changes along temperate rocky shores of the North Atlantic.

Ecological niche models, however, do not consider the species’ plastic and adaptive potential that could mitigate the predicted northward shifts. Adaptive potential depends largely on a population’s genetically effective size, N_e [34], or the size of an ideal population that undergoes the same rate of genetic change as the real population [35]. At low N_e, and low gene flow between populations, genetic drift generally plays the predominant role, effectively neutralizing selection, and eroding genetic diversity through stochastic fixation or loss of allelic variations [36–38]. Although N_e and temporal stability of genetic diversity patterns are particularly important for restoration and conservation efforts of fucoid seaweeds, only a single Norwegian population of F. serratus has so far been assessed [39].

The canopy-forming seaweed F. serratus is an excellent model for the study of temporal evolution and stability of genetic structure and diversity across a range of contrasting temperature regimes. It is one of the dominant intertidal seaweeds along the Northeast-Atlantic rocky shore from northern Portugal to northern Norway [40]. Arctic regions are predicted to become thermally suitable through 2100 under CO₂ emission scenario projections [26]. In contrast, regions south of the Brittany coast of France are predicted to become unsuitable [26], as temperatures will rise beyond the species’ potential for thermal acclimatization [41]. The susceptibility of F. serratus to climate change is expected to vary regionally, given the species’ regional patterns of genetic diversity [42], in combination with low gene flow between local populations [43].

Genetic diversity of F. serratus is highest in the two former, large glacial refugia (20–18 thousand years ago (kya)) in Southwest Ireland, and Brittany [42, 43]. The third refugium in the Northwest Iberian peninsula is characterized by a high proportion of private alleles, and currently represents the species’ isolated trailing edge, where recurrent cycles of thermally induced extinction and recolonization have eroded genetic diversity [42, 43]. Currently, sea surface temperatures reach 22 °C, and although below the lethal limit of F. serratus (25 °C) [40, 44], inhibit growth, physiological performance and reproductive capacity [45–48].

Genetic diversity of F. serratus decreases from its mid-range of distribution towards higher latitudes and is lowest in leading edge populations in northern Norway [42, 43]. Low genetic diversity in leading-edge populations is explained by the populations’ relatively young age and their derivation from small founder populations that carried only a subset of the genetic variation from glacial refugia to the north after the ice retreated, ca. 15–10 kya.

While N_e is a good indicator for temporal genetic stability, its estimation relies on temporally-spaced genetic data [49]. Due to this complication in sampling design, N_e of F. serratus has been estimated in only a single population close to Bergen (Norway) over eight years [39]. The estimated N_e between 73 and 386 was regarded insufficient for long-term survival under environmental change [39]. However, a thorough appraisal of the spatial distribution and temporal stability of N_e and genetic diversity throughout the species’ latitudinal range of distribution cannot be inferred from a single location.

Estimating climate change susceptibility in a species with low gamete/zygote dispersal requires to assess temporal genetic stability across its latitudinal and thermal range of distribution. In this study, we estimated N_e of F. serratus across latitude and temperature at seven locations with decadal sampling (2000 and 2010), a period in which Europe experienced three heat waves in 2003, 2006, and 2010 [50–52]. Here we evaluate whether range shifts in the north or strong selection pressures in the south have resulted in measurable changes in genetic diversity and population structure. In populations that are dominated by genetic drift and with small adaptive potential, we expected to find a decline in genetic diversity over the past decade. Finally, we discuss whether genetic diversity may be sufficiently stable to buffer environmental change and mitigate the current range shift predictions.

Methods

Sampling

Individuals were sampled ca. ten years apart from the same seven populations spanning the latitudinal distribution of F. serratus (Fig. 1). Ethical approval is not required for research work with the seaweed/macroalga F. serratus. Field collections did not require specific permits and the species is neither endangered nor protected. Sampling involved removing a thumbnail-sized piece of tissue from ca. 50 to 100 individuals at each sampling site and did not threaten either the individual or the local population. Live samples were never transferred to other countries or locations within any of the countries. In all cases the specimens were collected within the context of various grants (see funding information) that involved at least one of the co-authors and one or more colleagues from the country where the collection was made.

Fig. 1.

Sampling sites. Coordinates, years of collection, sampling sizes (n), and daily average sea surface temperatures (SST) at each of the seven sampling sites. SSTs were identical between the two Norwegian sampling sites as well as between the two French and the two Spanish sampling sites. Summer temperatures were exceptionally high at the Danish and Spanish sampling sites during the first two of three heat waves that Europe experienced in years 2003, 2006, and 2010

Variability in daily average sea surface temperatures and surface air temperatures at the sampling locations (Fig. 1, Additional file 1), recorded from 1999 to 2011, were extracted from the NOAA/OI/SST/V2 dataset (0.25° resolution, described in [53]) and the CPC Global Temperature dataset (0.5° resolution) provided by NOAA/OAR/ESRL/PSD, Boulder, Colorado, USA, [54]). Thermal variability was replicated in the two Norwegian, the two French, and the two Spanish samples, respectively. In Denmark, only a single population was sampled at two time points. Individual tissues were blotted dry and stored in silica prior to transport for subsequent DNA extraction.

Microsatellite genotyping

DNA was extracted from 2 mg silica dried tissue according to [55] with the modifications described in [56], followed by a purification step with the OneStep-96 PCR Inhibitor Removal Kit (Zymo Research, Irvine, USA) and a 1:3 dilution of the purified product. The samples were genotyped for a total of 31 microsatellite markers: 11 anonymous loci (L20, L38, L58, and L94 described in [57]; B113, B128, E6, E9, D39, A198, and F4 described in [58]) and 20 loci linked to expressed sequence tags (ESTs: F12, F22, F34, F36, F60, F45, F50, F17, F72, F49, F14, F21, F58, F19, F37, F65, F59, F69, F9, and described in [56]) (Additional file 2).

Polymerase chain reactions (PCRs) with 5 μl total volume contained 1 μl purified DNA template, 1.34 μl nuclease-free Water (Ambion, Thermo Fisher Scientific), 2.5 μl of AmpliTaq Gold 360 MM (Applied Biosystems, Life Technologies) and 0.08 μl of each forward and reverse primer (each primer at 20 μM; forward primer labeled with 6FAM, NED, PET or VIC; Applied Biosystems, Life Technologies). PCR was performed in a Veriti 96-Well Thermal Cycler (Applied Biosystems, Life Technologies). The conditions are depicted in Additional file 3 and specified for each marker in Additional file 2.

The fragment lengths were determined on an ABI 3500xl Genetic Analyzer from 1 μl of diluted PCR products (specified for each marker in Additional file 2) mixed with 8.9 μl of HiDi Formamide (Life Technologies) and 0.1 μl of Gene Scan 500 LIZ Size Standard (Life Technologies) after 5 min denaturation at 95 °C. Allele calling was performed with the GeneMapper v 4.1 Software (Applied Biosystems, Thermo Fisher Scientific).

Data analysis

The microsatellite raw dataset (Additional file 4) was corrected for allelic dropout with a Maximum Likelihood approach [59] using the program MicroDrop [60]. From the corrected data (Additional file 5), nine markers E9, F14, F17, F36, F37, F59, F60, F65, and L20 were removed from the full set of 31 markers before further analyses with the remaining 22 markers because the proportion of missing data for the excluded markers exceeded 12% in at least one of the populations.

Diversity estimates

Average locus heterozygosity H_exp (bias-corrected [61]), allelic richness α (the average number of alleles per locus) and multi-locus heterozygosity (MLH), the number of heterozygous loci per individual divided by the number of loci, were calculated for each sampling location. Regional estimates were obtained after pooling the two spatial samples from each of the Norwegian, Spanish and French regions. Regional estimates were not possible for the Danish region because only one population was sampled. H_exp was calculated with the R package ‘DEMEtics’ [62], and α was estimated with the R package ‘PopGenReport’. For local estimates, α was normalized to a sample size of 24, the smallest number samples in a population. For regional estimates α was normalized to a sample size of 24, and additionally, to a sample size of 50. MLH was estimated with the R package ‘InbreedR’. Inbreeding coefficients F_IS [63] were estimated with the R package ‘Demerelate’ and tests for significant deviation from 0 were based on 1000 iterations. We tested for significant temporal changes of H_exp, α, F_IS, and MLH at each sampling location with Wilcoxon rank sum tests in R [64]. To assess temporal evolution of diversity estimates, we tested for correlation between current and historical local measures with a Spearman’s rank correlation in R [64]. Additionally, we tested for significant differences between average present-day and historical values using Wilcoxon Rank Sum tests in R [64].

Effective population sizes (N_e) were estimated with an assumed generation time of 2 years [65] with the R package ‘NB’ after removing loci with only one allele: Locus F9 for the Kirkenes population, locus F72 for the Ribadeo1 population and loci F21 and F72 for the Ribadeo2 population.

Genetic differentiation

Population structure was determined with Bayesian clustering methods implemented in the software Structure v2.3.4 [66]. Acceptance of six clusters (K) was determined with the δ K Method [67] in the R package ‘pophelper’ [68].

Temporal genetic changes at each sampling location and geographic genetic differentiation within and between all historical and recent samples were estimated by the fixation index F_ST [69] using GENETIX 4.05 [70] and the differentiation index D_est [71]) using the R package ‘DEMEtics’ 0.8–7 [62]. D_est more correctly measures the true genetic differentiation compared with F_ST for multi-allelic markers such as microsatellites [62, 71]. Statistical significance of the pairwise comparisons was based on 10,000 permutations for F_ST and on 1000 Bootstrap repeats for D_est. To assess temporal stability of geographic differentiation, we tested for correlation between recent and historical F_ST and D_est values with Spearmans’s rank correlation in R [64]. Additionally, we tested for significant differences between average present-day and historical values using Wilcoxon Rank Sum tests in R [64]. Finally, we tested for correlation between temporal genetic differentiation (F_ST, D_est) and N_e with Pearson’s product moment correlation in R [64].

Results

Genetic structure

Bayesian clustering with the program Structure revealed clear differences between regions but not with time (Fig. 2). Historical and present-day F_ST values were strongly positively correlated (r = 0.93, p < 0.00001), and the overall historical F_ST value (0.21) did not differ significantly (p = 0.567) from the present-day value (0.22), indicating that spatial genetic differentiation between populations was globally consistent over time (Fig. 3a). Historical and present-day D_est values supported these findings as the overall values (0.40 and 0.42, respectively) did not differ significantly (p = 0.636) and were positively correlated (r = 0.97, p < 0.00001, Fig. 3b). Isolation by distance was indicated by stronger differentiation among than within countries (Additional file 6).

Fig. 2.

Clustering of samples. Sample assignment to six clusters (colors) with the program Structure shows consistent geographic differentiation between sampling times. Here, new and old refers to the two sampling years specified in Fig. 1

Fig. 3.

Present and historical genetic differentiation. Population differentiation estimated by F_ST (a) and D_est (b) with a 1:1 reference line

Temporal changes, however, were noted on a local level. Local differentiation between the Norwegian populations decreased from 2004 to 2010 (Additional file 6). All but the French population ‘Ile de Siec’ changed significantly in genetic variation over time, as indicated by significant changes in F_ST and D_est (Additional file 7). The Spanish population ‘Ribadeo2’ showed significant temporal change in F_ST but not in D_est.

Genetic variation/diversity

Stable population diversities through time were indicated by significant correlations of historical and present-day intra-population diversity indices (Fig. 4; H_exp: r = 0.86, p = 0.02, MLH: r = 1, p = 0.0004; α: r = 0.96, p = 0.003; F_IS: r = 0.82, p = 0.03). Moreover, average present-day values did not differ significantly (p > 0.05) from average historical values (H_exp: present = 0.56, historical = 0.56; MLH: present = 0.61, historical = 0.62; α: present = 6.22, historical = 6.36; F_IS: present = − 0.10, historical = − 0.10).

Fig. 4.

Genetic diversity across latitudes. Present and historical diversity estimates of a) multi-locus heterozygosity (MLH), b) allelic richness (α), c) expected heterozygosity (H_exp), and d) inbreeding (F_IS), with 1:1 reference lines representing unchanged temporal evolution

Local and regional diversity estimates (Additional file 8) were highest in France and lower at the northern and southern distribution edges (Fig. 4). Regional α estimates (standardized to 50 samples) exceeded local estimates (standardized to 24 samples) in all regions (Additional file 8).

Effective population size (N_e) was highest in the French population ‘Ile de Siec’ (N_e = 10,000,000) and lowest in the Norwegian population ‘Grense Jakobselv’ (N_e = 62) (Fig. 5, Additional file 7). N_e for the other populations ranged from 700 to 200 in the order: Gjerild Klint > Green Top > Ribadeo2 > Ribadeo1 > Kirkenes. At both sampling time points, none of the diversity estimates were significantly correlated with effective population size (all p > 0.09). The temporal decrease in MLH in Ribadeo2 was strong but not significant (p = 0.051, Additional file 7). The F_IS in ‘Kirkenes’ was significantly negative (p = 0.043, Additional file 8).

Fig. 5.

Effective population sizes across latitudes. Effective population size (N_e) at each sampling location with 95% confidence intervals

Discussion

The spatial distribution of genetic diversity has important implications for conservation and management as it reveals a species’ strong and weak points with regards to stability and evolutionary capacity [72–74]. Given the key ecological role of F. serratus [30–32, 75–77], the positive effect of genetic diversity may reach beyond the species level to affect community structure and increase species richness and stability of the entire associated ecosystem [78–81]. We are not aware of seaweed studies that have investigated positive ecosystem effects of genetic diversity, but genetic diversity enhanced heat-stress survival in germlings of Fucus vesiculosus [27]. Furthermore, in the habitat forming seagrass Z. marina, genotypic diversity not only enhanced biomass production, but also abundance of the associated fauna under near-lethal water temperatures [82] and community resistance to grazing [83]. Thus, maintaining genetic diversity in F. serratus is also expected to be important for conservation and management of the entire intertidal ecosystem along temperate rocky shores. Across the latitudinal range of F. serratus, genetic diversity and differentiation remained stable for 5–10 generations at regional scales, and in all but the Norwegian region at local spatial scales (Figs. 2, 3, 4). This suggests that, despite low gene flow between populations, effective population sizes have remained large enough to maintain genetic variation at least on the short term. Temporal genetic differentiation was systematically lower than local differentiation, and 1–2 orders of magnitude lower than regional differentiation (Additional file 9). This implies that temporal snapshots provide valuable information for conservation management of fucoid seaweeds, as they reliably reflect diversity and differentiation patterns for at least a decade.

N_e comparisons

In all but the Norwegian populations, N_e was estimated as > 260, a size reported as the median estimate for stable populations in over 83 studies spanning a diverse range of taxa [36]. This suggests low sensitivity to genetic stochasticity [36] in all but the northern edge populations of F. serratus. As in most studies, the precision of N_e decreased as N_e increased (Fig. 5) [36, 84, 85]. Local differentiation in F. serratus is one of the most important assumptions of the employed ‘temporal’ method to estimate N_e, in which neutral genetic change over time is expected to be inversely proportional to N_e. Discrete generations are another important assumption of the ‘temporal’ method. Overlapping generations are unlikely to cause a significant downward bias of N_e when more than 4 generations lie between the temporal samples [49]. This can be expected for most of our temporal samples, assuming a generation time of 1–2 years [65, 86] and a time span of 6–11 years between sampling. Thus, our sample-size-corrected estimates can be regarded as unbiased and indicative of a ‘real’ decline in N_e from the species’ mid-range of distribution to its range-edges.

An N_e > 1000, as in the French ‘Ile de Siec’ population, is large enough to ensure evolutionary potential in perpetuity [87], and is likely to provide the best source for adaptive genetic rescue of threatened and declining populations [38, 88]. However, large N_e estimates are commonly associated with a high uncertainty [36, 85]. Accordingly, the point estimate of N_e in the ‘Ile de Siec’ population (ca. 10 Million) has a wide confidence interval as compared with the other populations (Fig. 5, Additional file 7). Consequently, the point estimate is unlikely to be the true value in this population, but is certainly > 1000, and higher than in any other measured populations. The reason for this outlier value is not due to high diversity, since this is comparable to the other French population (Fig. 4a-d), but the high stability in allele frequencies over time. Indeed, the ‘Ile de Siec’ population was the only population for which temporal genetic differentiation was non-significant (Additional file 7).

N_e in the other mid-range populations, > 500, may be sufficient in the mid-term [36, 87, 89] to mitigate the predicted extinction by the end of the twenty-first century [26]. However, given that summer temperatures are predicted to rise above the thermal tolerance limit of F. serratus in Brittany within the next 200 years [26, 41], it is important to track its fitness in this region in order to implement early conservation measures in case it loses its current stability.

An N_e of 50–100 was regarded necessary for a population to minimize inbreeding depression and associated problems such as accumulation of deleterious mutations and loss of variation [36, 87]. However, despite N_e < 60 in the Norwegian ‘Grense Jakobselv’ population, genetic diversity remained stable for both Norwegian populations over six years and neither population was inbred. In contrast, a previous study on a southern Norwegian population reported significant loss of N_e from 2000 to 2008 and concluded that an N_e between 73 and 386 was insufficient for long-term survival under environmental change [39]. Stable diversity despite small N_e in our two northern Norwegian populations may be ascribed to regional gene flow, suggested by a reduction in genetic differentiation between the two Norwegian populations from 2004 to 2010 and significant outbreeding (negative F_IS) in the ‘Kirkenes’ population in 2010. Thus, regional gene flow may uncouple N_e from genetic stochasticity effects at the species’ Northern edge of distribution.

Diversity comparisons

As expected for neutral loci, genetic diversity was positively related with N_e [36, 38]. Both regional and local diversities are highest in Brittany and make the range-center of F. serratus less sensitive to genetic drift [36, 37]. A decline in genetic diversity towards the northern and southern range-edges is in accordance with the species’ biogeographic history [42].

Low genetic diversity does not necessarily lower the evolutionary potential of F. serratus to adapt to Arctic shores [26]. The evolutionary potential, survival, and expansion rate of low-diversity leading edge populations [3] may decrease when deleterious mutations accumulate at expansion range fronts and create a so-called ‘expansion load’ [7, 9]. On the other hand, survival may well be enhanced by the ‘surfing’ of favorable mutations that can rapidly increase in frequency over iterated founder events [4, 5, 90]. An additional consideration is that source populations of Arctic colonists may not be located at the species’ northern edge, but within European harbors with frequent shipping, fishing, and cruise boat traffic to and from the northern polar regions.

Our results have clear implications for the conservation of F. serratus at its southern edge. Reductions in MLH from 1999 to 2010 were close to significant (p = 0.0051/0.134 for Ribadeo2/Ribadeo1, respectively), although, H_exp, α, and F_IS remained temporally stable. This agrees with stability of H_exp and α over 7–9 years in fragmented southern edge populations of the kelp species Laminaria digitata [91], and is likely due to the measures’ insensitivity to the effects of population bottlenecks [92]. In other words, while the polymorphic state of loci and the diversity of alleles did not decline, alleles occurred more frequently in a homozygous state in the recent samples. In theory, the decline in MLH might be explained by increased selection pressure for heat-tolerance, although there is only indirect experimental evidence for this. Acclimation potential to further thermal stress is likely impeded in this population by chronically high expression of heat shock protein genes [22, 23, 41, 93]. Between 2000 and 2010, the Ribadeo1 population experienced a 90% decline in abundance [26]. Although stable local differentiation favors ecotypic differentiation in thermal stress tolerance [41], heat-stress is becoming too extreme at the southern edge.

The value of conserving the southern edge of F. serratus may be high [94]. Because of its separation from Brittany by the uninhabitable sandy warm shores of the Bay of Biscay, the Northwest Iberian glacial refugium did not contribute to postglacial recolonizations of ice-free northern shores, and, thus, preserves unique genetic variation [42]. The conservation value of the species’ southern edge becomes even more apparent when considering that small-scale population structure increases the species’ regional diversity above local diversity within single populations (Additional file 8). High regional diversity, despite low within-population diversity, was previously reported for the southern distribution edge of the seagrass Zostera marina [94, 95]. We are not aware of studies that explicitly addressed this effect in macroalgae, although increased local differentiation at the southern edge of the kelp Laminaria digitata [91] can be expected to increase regional variation as well. Thus, with the loss of its southern edge, the species’ can be expected to lose its most heat-adapted populations sustaining unique genetic variation.

Conclusions

Temporal snapshots of genetic diversity and structure in F. serratus populations spanning its latitudinal range reliably reflect patterns across local and regional spatial scales and across various thermal backgrounds for at least one decade. Stable small-scale structure enhances regional genetic diversity throughout the species’ range of distribution and is a potential driver for local adaptation [36] that may explain species radiation and diversity in the genus Fucus [96–98].

MLH appears to be the most stress-sensitive measure of diversity, displaying a strong decline at the species’ southern edge of distribution. As sandy warm shores separate the Iberian southern edge from the genetically diverse Brittany region, genetic rescue by the influx of genetic variation [38, 88] might only be possible if initiated by conservation efforts.

Increasing temperatures are likely the major cause for the decline not only of F. serratus, but also other intertidal and subtidal macroalgae in Northwest Iberia [28, 99–101], as well as temperate seaweeds worldwide [102]. Kelp species may maintain genetic diversity to a certain degree in southern edges by escaping to deep-water refugia to avoid rising temperatures in shallow waters [103]. Accordingly, in Northern Portugal, increasing air-temperature stress depresses the upper boundary limit of F. serratus [104]. However, intertidal seaweeds are less adapted to low light conditions and, thus, have low potential to escape into deeper waters. Another factor that impedes survival of southern edge populations in fucoid seaweeds is their reproductive strategy with fewer gametes and lower dispersal (< 12 m from parental sites [45, 46]) as compared with kelps that release billions of spores dispersing several kilometers [105, 106]. We suspect that without the influx of genetic variation from Brittany, intertidal habitat-forming macroalgae, such as F. serratus, may largely disappear from southern edges but retain potential to persist in small subtidal bottleneck populations in cool upwelling regions [107].

Abbreviations

CI

Confidence interval

D_est

Differentiation index measuring genetic differentiation

DNA

Deoxyribonucleic acid

EST

Expressed sequence tag

F_IS

Inbreeding coefficient

F_ST

Fixation index measuring genetic differentiation

H_exp

Expected heterozygosity

kya

thousand years ago

MLH

Multi locus heterozygosity

N_e

Effective population size

PCR

Polymerase chain reaction

SAT

Surface air temperature

SST

Sea surface temperature

α

Allelic richness

Authors’ contributions

All authors were involved in sample collection, project planning, and experimental design. AJ, GH, and JAC performed the laboratory work. AJ analyzed the data and wrote the manuscript. All authors read and approved the final manuscript.

Ethics approval and consent to participate

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.