Figure 4. Inhibition SFKs leads to cell cycle arrest but does not trigger efficient apoptosis.

(A–D) NSCLC cell lines were treated with the indicated concentrations of dasatinib (Das) or PP2 for 24 h and samples subjected to Edu staining to assess DNA synthesis (A), SDS-PAGE/Western blotting for the indicated cell cycle regulators (B) and apoptotic markers (D) such as cleaved (cl) caspases and caspase 3/7 activity assays (C). Results shown are either representative (B and D) or the average of of a minimum of three independent experiments performed in quadruplicate. Statistical analysis: unpaired two-tailed Student's t-test with Welch correction (^*p < 0.05, ^**p < 0.01, ^***p < 0.005).