Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Schizosaccharomyces pombe) |
Mto1 | NA | Pombase:SPCC417.07c | |
| Gene (S. pombe) | Mto2 | NA | Pombase:SPBC902.06 | |
| Gene (S. pombe) | Crm1 | NA | Pombase:SPAC1805.17 | |
| Gene (S. pombe) | Nup146 | NA | Pombase:SPAC23D3.06c | |
| Gene (S. pombe) | Alp7 | NA | Pombase:SPAC890.02c | |
| Gene (S. pombe) | Nup132 | NA | Pombase:SPAC1805.04 | |
| Gene (S. pombe) | Spi1 | NA | Pombase:SPBC1289.03c | Spi1 is the S. pombe gene name for Ran GTPase |
| Gene (S. pombe) | Plo1 | NA | Pombase:SPAC23C11.16 | |
| Gene (S. pombe) | Alp14 | NA | Pombase:SPCC895.07 | |
| Gene (Saccharomyces cerevisiae) |
Nup159 | NA | SGD:S000001377 | Alternate systematic name YIL115C |
| Gene (Homo sapiens) |
Nup214 | NA | HGNC:8064 | Alternate name CAN |
| Antibody | TAT1 anti-tubulin (mouse monoclonal) |
PMID:2606940 | (1:15 of hybridoma supernatant) |
|
| Antibody | Alexa Fluor 488 secondary (donkey anti-mouse polyclonal) |
Thermo Fisher Scientific | Thermo Fisher Scientific :A-21202; RRID:AB_141607 |
(1:80) |
| Antibody | anti-GFP (rabbit polyclonal) | Rockland | Rockland:600-401-215; RRID:AB_828167 |
(1:400) |
| Antibody | Alexa 594 FluoroNanogold Fab’ fragment (goat anti-rabbit polyclonal) |
Nanoprobes | Nanoprobes:7304 | (1:400) |
| Antibody | anti-GFP (sheep polyclonal) | other | homemade lab stock; used for immunoprecipitation |
|
| Commercial assay or kit |
QuikChange II Site Directed Mutagenesis Kit |
Agilent | Agilent:200523 | |
| Chemical compound, drug |
Leptomycin B | LC Laboratories | LC Laboratories: L-6100 |
|
| Software, algorithm |
Metamorph | Molecular Devices | RRID:SCR_002368 | |
| Software, algorithm |
Image J | NIH | RRID:SCR_003070 | |
| Software, algorithm |
Prism | Graphpad | RRID:SCR_002798 | |
| Software, algorithm |
Photoshop | Adobe | RRID:SCR_014199 | |
| Software, algorithm |
Illustrator | Adobe | RRID:SCR_010279 | |
| Software, algorithm |
MaxQuant | PMID:19029910 | RRID:SCR_014485 | |
| Software, algorithm |
MaxLFQ | PMID:24942700 | ||
| Software, algorithm |
Pombase | PMID:25361970 | RRID:SCR_006586 | |
| Other | DAPI | Thermo Fisher Scientific | Thermo Fisher Scientific: D1306; RRID:AB_2629482 |
|
| Other | Protein G Dynabeads | Thermo Fisher Scientific | Thermo Fisher Scientific: 10003D |
|
| Other | Fractogel EMD Chelate (M) | Merck KGaA | Merck:1.10338.0010 | |
| Other | Nanolink streptavidin magnetic beads |
Trilink | Trilink:M-1002 |