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The Scientific World Journal logoLink to The Scientific World Journal
. 2002 Jun 18;2:1662–1666. doi: 10.1100/tsw.2002.846

Purification of Lipid Rafts from Cultured Cells

John Graham 1,*
PMCID: PMC6009391  PMID: 12806159

Abstract

Lipid-rich lipid rafts are microdomains of the plasma membrane that are resistant to low concentrations of nonionic detergent. This forms the basis for their isolation. Either a microsomal fraction or a postnuclear supernatant are loaded beneath a discontinuous iodixanol gradient. If all the solutions contain 0.5–1.0% Triton X-100, the intact lipid rafts float to the top of the gradient while all of the other detergent-solubilized membranes remain at the bottom.

Keywords: lipid rafts, membrane vesicles, plasma membrane, cultured cells, detergent-resistant membranes, Triton X-100, OptiPrepTM, iodixanol, discontinuous gradient


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