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. 2018 May 29;7:e33092. doi: 10.7554/eLife.33092

Figure 1. Rem2 expression is developmentally regulated and activity-dependent.

Figure 1.

(A) (left) Western Blot of cortical lysate from rat brains at different developmental ages detected using anti-Rem2 (1:500); anti-βactin (1:5000) was used as a loading control. (right) Quantification of relative Rem2 intensity at different developmental ages normalized to anti-βactin. Molecular Weight of REM2: ~50 kDa. Molecular weight of ACTB: 42 kDA. (B) Fold change in Rem2 (left) or Fos mRNA expression (right) in isolated primary visual cortex (V1) or somatosensory cortex (S1) from P28 mice raised in the dark (DR, from P9-P28) or raised in the dark (P9–P28) and exposed to light for 90 m (90 m Light). N = 3 biological replicates of 4 mice in each experiment. mRNA levels were normalized to Actb levels and then to S1 DR condition and presented as mean ± SEM. *p<0.05 from V1 DR by one-way ANOVA followed by a Dunnett’s post hoc test.