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. 2018 Jun 19;5(6):1870036. doi: 10.1002/advs.201870036

Volumetric Nanoscale Imaging: Hard X‐Ray Nanoholotomography: Large‐Scale, Label‐Free, 3D Neuroimaging beyond Optical Limit (Adv. Sci. 6/2018)

Anna Khimchenko 1, Christos Bikis 1, Alexandra Pacureanu 2, Simone E Hieber 1, Peter Thalmann 1, Hans Deyhle 1, Gabriel Schweighauser 3, Jürgen Hench 3, Stephan Frank 3, Magdalena Müller‐Gerbl 4, Georg Schulz 1, Peter Cloetens 2, Bert Müller 1
PMCID: PMC6010702

Abstract

In article number https://doi.org/10.1002/advs.201700694, Bert Müller and co‐workers launch nano‐holotomography for the isotropic 3D imaging of paraffin‐embedded human brain tissues. Nano‐holotomography bridges the spatial resolution gap between optical and electron microscopy, while giving access to 3D data for large tissue volumes with a spatial resolution well below 100 nm. The hierarchical approach will mediate prospering nano‐anatomy.

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Keywords: cerebellum, hierarchical imaging, human brain, neocortexes, neuroimaging, segmentation


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