Fig. 1.

WW437 is a novel HDACi. (a) Schematic diagram of the screening strategy. (b) Chemical structure of SAHA and WW437. (c) A panel cell lines (MDA231, 4 T1, BT549 and MCF10A) were treated with indicated concentrations of WW437. After 48 h, the MTS assay was performed. The bars indicate the mean ± SD. (d) Acetylated histone H3 (Ac-H3) and Acetylated histone H4 (Ac-H4) protein level was induced by WW437 in various breast cancer cell lines. SAHA was used as a positive control (1 µM). Actin was used as loading control. (e) MDA231 cells were seeded on coverslips. Cells were exposed to WW437 for 24 h, and then fixed, and stained with the indicated antibodies. Scale bar, 40 μm. (f) Cells were treated with different concentrations of WW437 for 24 h and then whole cell lysates were collected. Western blot assays were performed using indicated antibodies.