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. Author manuscript; available in PMC: 2018 Jun 25.
Published in final edited form as: Virology. 2015 May 15;483:149–162. doi: 10.1016/j.virol.2015.04.002

FIG. 6.

FIG. 6

Replication of SELEX winners in protoplasts. (A) Northern blot analysis of RNA extracted from protoplasts at 40 hpi. Protoplasts were inoculated with wt satC, negative control (unSELEXed) satCs with various length H2 regions (sequences listed below the blots), or satC SELEX winners. A set of three representative blots from one of three independent experiments is shown. Each blot contained a wt satC control for normalization purposes; normalization to the rRNA levels in the EtBr stained gel also was performed. The positions of satC monomers and dimers, as well as the length of the H2 region, are indicated to the left and below the figure, respectively. (B) Quantitation of monomers and (C) dimers from the replicate experiments. Standard deviations are indicated. (D) Dimer:monomer ratio from one set of blots.