Table 2.
Regression equation, LOD and LOQ, precision, repeatability, stability, and recovery of 21 investigated compounds.
| No. | Compounds | Regression Equation | r2 | Liner Range (μg/mL) | LOD (ng/mL) | LOQ (ng/mL) | Precision RSD (%) | Repeatability RSD (%) (n = 6) | Stability RSD (%) | Recovery (%) (n = 3) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Intar-Day (n = 6) | Inter-Day (n = 6) | Low | Medium | High | ||||||||||||
| Mean | RSD | Mean | RSD | Mean | RSD | |||||||||||
| 1 | Catalpol | Y = 20.5X + 888 | 0.9997 | 0.015–7.600 | 1.52 | 5.32 | 2.22 | 3.70 | 3.68 | 1.44 | 98.67 | 1.62 | 98.34 | 2.74 | 99.26 | 3.35 |
| 2 | Aucubin | Y = 9.05X + 13,800 | 0.9997 | 0.130–195.000 | 3.90 | 13.65 | 3.45 | 2.10 | 2.87 | 1.16 | 99.00 | 2.27 | 99.16 | 1.78 | 98.77 | 2.10 |
| 3 | Harpagide | Y = 211X + 2990 | 0.9999 | 0.017–8.320 | 1.44 | 3.98 | 1.41 | 2.93 | 3.65 | 3.73 | 97.62 | 3.14 | 97.47 | 3.98 | 97.56 | 3.68 |
| 4 | Geniposidic acid | Y = 1060X + 48,400 | 0.9999 | 0.025–127.200 | 1.27 | 4.45 | 2.15 | 2.62 | 2.09 | 3.85 | 99.91 | 3.08 | 98.43 | 1.76 | 97.56 | 2.55 |
| 5 | Neochlorogenic acid | Y = 2130X + 26,000 | 0.9999 | 0.020–9.800 | 1.96 | 6.86 | 1.45 | 2.40 | 2.05 | 1.34 | 99.77 | 3.67 | 96.45 | 3.81 | 98.01 | 2.15 |
| 6 | Protocatechuic acid | Y = 3210X + 171,000 | 0.9996 | 0.013–12.520 | 1.25 | 4.38 | 2.08 | 3.39 | 3.23 | 1.86 | 95.83 | 3.09 | 99.05 | 3.40 | 97.01 | 3.55 |
| 7 | Syringin | Y = 115X + 7860 | 0.9997 | 0.019–19.440 | 1.94 | 6.80 | 2.96 | 2.23 | 3.56 | 1.24 | 98.38 | 2.29 | 100.16 | 2.82 | 98.81 | 2.82 |
| 8 | Chlorogenic acid | Y = 170X + 176,000 | 0.9996 | 0.238–476.000 | 4.76 | 16.66 | 1.88 | 2.61 | 1.57 | 2.76 | 98.20 | 2.64 | 98.24 | 2.19 | 97.59 | 2.85 |
| 9 | Pinoresinol-di-O-β- d-glucopyranoside | Y = 423X + 15,200 | 0.9998 | 0.150–75.000 | 3.75 | 13.13 | 1.09 | 3.07 | 2.83 | 3.20 | 98.29 | 1.20 | 98.01 | 2.92 | 96.72 | 3.88 |
| 10 | Geniposide | Y = 48.3X + 36,700 | 0.9991 | 0.080–40.200 | 1.47 | 4.94 | 1.20 | 2.16 | 1.45 | 2.64 | 98.20 | 3.85 | 96.45 | 1.17 | 96.99 | 3.52 |
| 11 | Cryptochlorogenic acid | Y = 350.5X + 506,010 | 0.9996 | 0.119–118.800 | 2.97 | 10.40 | 2.04 | 3.57 | 1.94 | 1.63 | 96.83 | 2.08 | 96.13 | 3.31 | 98.34 | 3.19 |
| 12 | Caffeic acid | Y = 2540X + 114,000 | 0.9998 | 0.024–11.920 | 1.19 | 4.17 | 1.87 | 2.91 | 2.74 | 3.84 | 98.38 | 3.68 | 96.95 | 3.37 | 100.43 | 3.07 |
| 13 | Rutin | Y = 652X + 238,000 | 0.9999 | 0.135–67.700 | 0.60 | 1.99 | 1.39 | 3.58 | 1.54 | 3.57 | 98.15 | 3.82 | 97.28 | 3.45 | 95.48 | 2.96 |
| 14 | Isoquercitrin | Y = 2640X + 216,000 | 0.9995 | 0.117–58.400 | 0.89 | 3.02 | 2.15 | 3.29 | 2.09 | 2.54 | 98.16 | 3.55 | 96.59 | 2.08 | 102.03 | 3.16 |
| 15 | Genipin | Y = 953X + 68,600 | 0.9991 | 0.034–33.500 | 0.67 | 2.35 | 3.26 | 3.14 | 2.68 | 3.30 | 98.41 | 2.29 | 96.34 | 3.44 | 98.09 | 3.55 |
| 16 | Pinoresinol-4’-O-β- d-glucopyranoside | Y = 0.89X +116 | 0.9991 | 0.022–11.180 | 1.12 | 3.91 | 2.70 | 2.40 | 3.60 | 2.99 | 102.35 | 3.29 | 97.18 | 2.95 | 97.43 | 2.13 |
| 17 | Astragalin | Y = 3050X + 130,000 | 0.9999 | 0.027–13.440 | 0.98 | 2.96 | 2.07 | 3.04 | 2.55 | 2.80 | 99.89 | 3.87 | 98.52 | 1.99 | 96.71 | 2.18 |
| 18 | Isochlorogenic acid A | Y = 1300X + 149,000 | 1.0000 | 0.090–44.800 | 2.24 | 7.84 | 2.99 | 3.07 | 3.31 | 3.59 | 97.34 | 3.49 | 97.69 | 2.58 | 97.07 | 2.98 |
| 19 | Kaempferol | Y = 429X + 1010 | 1.0000 | 0.026–13.000 | 2.36 | 5.90 | 1.87 | 2.16 | 3.77 | 3.36 | 96.13 | 3.15 | 96.85 | 2.93 | 97.92 | 2.04 |
| 20 | Baicalein | Y = 531X − 1080 | 0.9991 | 0.0015–7.740 | 0.32 | 1.15 | 2.68 | 3.08 | 3.04 | 3.57 | 97.62 | 3.52 | 103.30 | 2.38 | 98.64 | 3.38 |
| 21 | Quercetin | Y = 2.46X + 123,000 | 0.9991 | 0.126–62.950 | 1.45 | 4.69 | 2.14 | 3.76 | 1.02 | 3.38 | 95.91 | 3.41 | 98.65 | 3.73 | 97.45 | 2.03 |