Figure 2. The VDA-derived LCs are reduced in Runx1 and cMyb mutants.

(A) Anti-GFP staining shows that the VDA-derived LCs are significantly reduced in 12 dpf runx1^W84X mutants comparing with those in siblings. (B) Quantification of the density of the VDA-derived GFP⁺ LCs in 12 dpf runx1^W84X mutants and siblings. n = 5 for siblings and n = 6 for mutants. Error bars represent mean SEM. **p<0.01. (C) Anti-GFP staining reveals a reduction of the VDA-derived LCs in 20 dpf cmyb^hkz3 mutants comparing with size matching siblings. (D) Quantification of the density of the VDA-derived GFP⁺ LCs in 20 dpf cmyb^hkz3 mutants and size matching siblings. n = 11 and 10 for siblings and mutants respectively. Error bars represent mean SEM. **p<0.01.

Figure 2—figure supplement 1. Quantification of body length and RBI derived LCs in Runx1 and cMyb mutants.

(A) Average body length of the runx1^W84X mutant and its sibling. n = 5 for siblings and n = 6 for mutants. Error bars represent mean SEM. (B) Average body length of the 20 dpf cmyb^hkz3 mutant and its size matched sibling. n = 11 and 10 for siblings and mutants respectively. Error bars represent mean SEM. (C) Quantification of the density of the RBI-derived GFP⁺ LCs in the trunk of 12 dpf runx1^W84X mutants and siblings. n = 6 for siblings and mutants. Error bars represent mean SEM. (D) Quantification of the density of the RBI-derived GFP⁺ LCs in the trunk of 20 dpf cmyb^hkz3 mutants and size matched siblings. n = 5 for siblings and n = 6 for mutants. Error bars represent mean SEM. (E) Quantification of the density of total LCs in the trunk of 12 dpf runx1^W84X mutants and siblings. n = 5 for siblings and n = 6 for mutants. Error bars represent mean SEM. (F) Quantification of the density of total LCs in the trunk of 20 dpf cmyb^hkz3 mutants and size matched siblings. n = 11 and 10 for siblings and mutants, respectively. Error bars represent mean SEM.