Table 4.
Chromatographic data, retention factor of first eluted enantiomer (k1), separation factor (α), resolution (RS), and elution order, for the chiral xanthonic analytes on the ChirobioticTM TAG column, under multimodal chromatographic conditions.
| Analyte | Elution Mode | Mobile Phase | k1 | α | RS | First Eluted Enantiomer |
|---|---|---|---|---|---|---|
| 1 | POM | 100% EtOH | 1.26 | 1.24 | 0.60 | (R) |
| 2 | NPM | Hex:EtOH (70:30 v/v) | 5.01 | 1.42 | 1.45 | (R) |
| POM | 100% EtOH | 0.73 | 1.39 | 0.80 | (R) | |
| 4 | NPM | Hex:EtOH (70:30 v/v) | 10.66 | 1.38 | 1.76 | (R) |
| POM | 100% EtOH | 1.21 | 1.36 | 0.92 | (R) | |
| 5 | NPM | Hex:EtOH (70:30 v/v) | 8.29 | 1.37 | 1.02 | (R) |
| 17 | NPM | Hex:EtOH (70:30 v/v) | 0.80 | 2.77 | 3.87 | (S) |
| 30 | NPM | Hex:EtOH (70:30 v/v) | 2.01 | 1.45 | 1.16 | (R) |
| 31 | NPM | Hex:EtOH (70:30 v/v) | 1.57 | 1.27 | 0.50 | (R) |
Chromatographic conditions: flow rate: 0.2 mL/min, UV detection at 254 nm.