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. 2018 Jan 5;23(1):75. doi: 10.3390/molecules23010075

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Distinct morphological changes in MG-63 after 48 h of treatment with DK1 (IC₂₅ (6.6 µM), IC₅₀ (23.8 µM), and IC₇₅ (30 µM)); (B) Distinct morphological changes in U-2OS after 48 h treatment with DK1 (IC₂₅ (2.2 µM), IC₅₀ (19.6 µM) and IC₇₅ (30 µM)); (C,D) Quantification analysis of MG-63 and U-2OS based on cells uptake of acridine orange and propidium iodide in 200 cells. (Blue arrow: viable; red arrow: apoptosis). EA (early apoptosis), LA (late apoptosis). All data are expressed as mean ± standard error mean (S.E.M). * p < 0.05 compared with corresponding controls (Magnification: 200×).

(A) Distinct morphological changes in MG-63 after 48 h of treatment with DK1 (IC₂₅ (6.6 µM), IC₅₀ (23.8 µM), and IC₇₅ (30 µM)); (B) Distinct morphological changes in U-2OS after 48 h treatment with DK1 (IC₂₅ (2.2 µM), IC₅₀ (19.6 µM) and IC₇₅ (30 µM)); (C,D) Quantification analysis of MG-63 and U-2OS based on cells uptake of acridine orange and propidium iodide in 200 cells. (Blue arrow: viable; red arrow: apoptosis). EA (early apoptosis), LA (late apoptosis). All data are expressed as mean ± standard error mean (S.E.M). * p < 0.05 compared with corresponding controls (Magnification: 200×).