Table 1. Summary of the deacetylation turnover (V/[E]) from data in Figure 6 and Figure 6—figure supplement 1 (p<0.001 for V/[E] of K9ac nucleosome vs. K14ac nucleosome substrates in both the monoacetylated and triacetylated assays); (n = 3 for all measurements except n = 2 for LHC on H3 and HDAC1 on nucleosomes; kinetic values shown are ± S.E.M.
| Substrate | V/[E] (min−1) | |||
|---|---|---|---|---|
| K9ac | K14ac | K18ac | ||
| LHC | Kac Nucleosome | 0.10 ± 0.0084 | 0.014 ± 0.0073 | 0.048 ± 0.011 |
| Kac H3 | 31 ± 3.9 | 35 ± 8.5 | - | |
| 3Kac Nucleosome | 0.11 ± 0.0071 | 0.019 ± 0.0033 | 0.058 ± 0.0022 | |
| 3Kac H3 | 31 ± 4.3 | 35 ± 2.7 | - | |
| HDAC1 | Kac Nucleosome | <0.005 | <0.005 | - |
| Kac H3 | 10 ± 0.89 | 12 ± 0.26 | - | |