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. 2018 Jun 26;9(3):e00567-18. doi: 10.1128/mBio.00567-18

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Copyright © 2018 Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 9 — Working model of single-molecule CRISPR-Cas9 transformation in S. sclerotiorum. The top half of the diagram illustrates the transformation and vector sequence integration events as described in the text. The bottom half of the diagram illustrates the deletion of tandem, direct repeat vector sequences by intrachromosomal recombination during meiosis following self-mating. NHEJ, nonhomologous end joining; DSB, double-stranded break; NLS, nuclear localization sequence; PAM, protospacer adjacent motif; Hph, hygromycin B phosphotransferase; gRNA, guide RNA; Cas9, CRISPR-associated protein 9; RNP, ribonucleoprotein.