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PLOS ONE logoLink to PLOS ONE
. 2018 Jun 28;13(6):e0199630. doi: 10.1371/journal.pone.0199630

Genetic combining ability of coriander genotypes for agronomic and phytochemical traits in response to contrasting irrigation regimes

Amir Gholizadeh 1,#, Hamid Dehghani 1,‡,*, Mostafa Khodadadi 1,#, Patrick J Gulick 2,
Editor: David A Lightfoot3
PMCID: PMC6023167  PMID: 29953470

Abstract

Knowledge of genetic combining ability and gene action would help breeders to choose suitable parents and devise an appropriate breeding strategy for coriander. In the present study, six diverse genotypes of coriander, their 15 F1s and 15 F2s were evaluated through randomized complete block design with three replications to study genetic combining ability for agronomic and phytochemical traits in coriander. Plants were subjected to well-watered (WW), mild water-deficit stress (MWDS) and severe water-deficit stress (SWDS) irrigation regimes. The results indicate that water-deficit stress decreased all of the measured traits in both the F1 and F2 generations. General combining ability and specific combining ability effects were highly significant for all of the traits in both the F1 and F2 generations. Additive gene action was predominant for phonology and fruit yield component traits in all irrigation regimes in both the F1 and F2 generations. For fatty acid content and total lipid yield, non-additive gene action was predominant in the F1 generation while additive gene action was predominant in the F2 generation under MWDS and SWDS conditions. The P4 parent had the highest general combining ability for fruit yield components in both the F1 and F2 generations. The P6 parent had the highest general combining ability for phenological and phytochemical traits. The P4 and P6 parents are promising material to develop early flowering and early maturing genotypes coupled with high total lipids in advanced generations of segregation.

Introduction

Coriander (Coriandrum sativum L.) is an annual herb that belongs to the umbelliferous plant family, the Apiaceae. The rapid life cycle of some coriander genotypes allow them to be cultivated in the wide range of geographical areas throughout the world [1]. Fresh and dried leaves and seeds are commonly used as a seasoning and a general food ingredient [2]. Coriander is mainly cultivated for its fruit characteristics that are used for different applications in the food, drug, cosmetic, and perfume industries [3]. Coriander fruit contains oils with a high concentration of monounsaturated fatty acids [4, 57]. The oils with different fatty acid compositions are important for human consumption and for industrial uses; oleic, linoleic and petroselinic acids are the main components of fatty acids in coriander. Oils with a high proportion of oleic acid are more stable than other vegetable oils and they are recommended in the diet to reduce the risk of cardiovascular diseases in humans [8]. On the other hand, linoleic acid is preferred by industries when oil hydrogenation is required and it is an essential fatty acid in the human diet. Petroselinic acid can be broken down into adipic (C6) and lauric (C12:0) acids by oxidative cleavage. Adipic acid is used for the manufacture of a wide range of polymers including high-grade engineering plastics. Lauric acid is used as a raw material for soaps, emulsifiers, detergents, and softeners [9].

The development of new crops for the production of industrial oils is an area of significant interest both scientifically and environmentally [4]. The quantity and composition of fatty acids may be affected by growth conditions, including water-deficit stress which can lead to change in the morphology, physiology and biochemistry of plants [1012]. There are several evidences that water-deficit stress can significantly decrease the fatty acid content and yield in plants such as safflower (Carthamis tinctorius L.) [13], sage (Salvia officinalis L.) [11], cumin (Cuminum cyminum L.) [14] and soybean (Glycine max L.) [15]. Therefore, development of drought-tolerant cultivars with a high total lipid yield is an important area of research in medicinal and industrial plants such as coriander.

Drought tolerance is defined as the ability of plants to live, grow and produce yield under water-deficit stress conditions [16]. Several studies have reported that the water-deficit stress can lead to reduced oil content and yield in oil seed and culinary seed crops including coriander [17, 18], dill (Anethum graveolens L.) [19], Plantago ovata and Nigella sativa [20], caraway (Carum carvi L.) [21], purple basil (Ocimum basilicum L.) [22] and cumin [23]. Yield is a complex quantitative trait that is affected by various phenological and yield component traits, each with its own genetic systems. The component traits can also be used as surrogate traits to assess drought tolerance and to identify genotypes with high yield potential for use in coriander improvement programs. Flowering time and maturity traits are known as the important factors in determining yield, moreover these traits can easily be evaluated by simple observation under field conditions [24]. Amiri-Oghan et al. [25] noted that days to early flowering and late maturity can be used as suitable indicators to screen for high yielding oilseed rape (Brassica napus L.) genotypes under water restricted conditions. Khodadadi et al. [26] showed that there was a significant negative correlation between days to flowering and fruit yield in coriander under water-deficit stress and that early flowering, a component of drought escape, enhanced fruit productivity in coriander under water-deficit conditions. Yield components can also be used as indicators for identifying the high yielding genotypes due to their ease of measurement and high heritability. When traits are governed by similar genetic control mechanisms these traits could simultaneously be improved by selection under water-deficit conditions [18].

Knowledge of the extent and nature of the genetic architecture and heritability of the major traits associated with yield and correlations between traits are essential to improve the efficiency of breeding programs [27]. The diallel mating design has been used to quantify the nature of gene action which control traits and also to estimate general combining ability (GCA) and specific combining ability (SCA) of parents and crosses, respectively. Examples of its use in a wide array of crops include those of Gao et al. [28] in Agaricus bisporus, Townsend et al. [29] in Artemisia annua, Zhang et al. [30] in barley, Khodadadi et al. [18] in coriander, dos Santos et al. [31] in Theobroma cacao, Pereira et al. [32] in cacao and Hirut et al. [33] in potato. GCA is defined as the average yield of a parental genotype over relevant hybrids and corresponds to additive genetic effects, while the SCA is defined as the yield of a hybrid that deviates from what would be expected if traits were controlled by additive effects alone, i.e. it represents non-additive genetic effects [34].

There is insufficient knowledge of the genetic control of phenological and yield component characteristics in coriander. The objectives of this study were (1) to quantify heritability and the nature of gene action controlling phonological traits, yield components and total lipid yield traits, and (2) to estimate genetic combining ability of parents and hybrids.

Materials and methods

Plant material and growth conditions

The coriander genotypes used to make diallel crosses had been evaluated in a preliminary experiment for drought tolerance by Khodadadi et al. [35]. Parents included the commercial genotype (P1), TN-59-353 (P2; relatively drought tolerant), TN-59-80 (P3; drought susceptible), TN-59-160 (P4; drought tolerant and relatively high yielding), TN-59-158 (P5; highly drought susceptible) and TN-59-230 (P6; highly drought tolerant but low yielding). All six parents were used in half diallel mating design, without reciprocals, to produce 15 F1 hybrids in 2014. Seeds of these F1 hybrids were used to produce 15 F2 generations through self-pollination in isolated conditions. All of the six parents, the F1 hybrids and F2 populations were evaluated in different irrigation regimes in experiments with a randomized complete block design with three replications in each experiment during growing season of 2016. Tests were carried out at the research field of Tarbiat Modares University in Iran (51° 09 ʹE longitude and 35° 44ʹ N latitude, at an elevation of 1265 m above sea level). In treatment 1, genotypes were kept well-watered overall (WW). In treatment 2, genotypes were well-watered until the commencement of stem elongation when watering was withdrawn until the end of the flowering stage at which time only one recovery watering was applied (mild water-deficit stress; MWDS). In treatment 3, watering was normal until the commencement of the flowering stage, after which watering was cut off completely (severe water-deficit stress; SWDS). The soil’s physical and chemical characteristics in the experimental field are presented in S1 Table.

Trait measurements

The traits which were measured included days to flowering (DTF), days to the end of flowering (DTEOF), days to ripening (DTR), umbel number per plant (UNPP), fertile umbel number per plant (FUNPP), fruit number per plant (FNPP), thousand fruit weight (TFW), fatty acid content (FAC) and total lipid yield (TLY). The timing of phenological traits were noted at the time at which 50% of plants in each plot had reached the target phonological stage. Sample size to measure yield components varied with genetic material; FAC and TLY traits were measured in ten plants in each plot for parental genotypes and F1 hybrids and in 30 plants in each plot for F2 populations.

To measure fatty acid content, two grams of powdered fruit samples of coriander were subjected extraction with a Soxhlet apparatus with 250 ml of petroleum ether for 6 h. Fatty acid content was measured after filtration and solvent evaporation under reduced temperature and pressure [18]. Finally, total lipid yield was estimated by multiplying fatty acid content with fruit yield per plant (g) for each plot.

Statistical analysis

The datasets were first tested for normality according to the Shapiro-Wilk test method [36]. The analysis of variance for GCA and SCA effects was done according to Griffing’s [37] method 2, model 1 using a SAS program proposed by Zhang et al. [38]. Mean values of the traits in different irrigation regimes were compared using least significant difference (LSD) method. Estimates of σg2 (general combining ability variance) and σs2 (specific combining ability variance) were computed based on the random-effects model of Griffing’s [37] method. These estimates were used to calculate σA2 (additive variance), σD2 (dominance variance), h2 (heritability), and the GCA/SCA ratio [38]. The relative importance of variances due to GCA and SCA were computed for the traits using the method proposed by Baker [39] (Eq 1).

GCA/SCAratio=2σg22σg2+σs2 (1)

The GCA/SCA ratio reflects the degree of trait transmission from parent to the progeny. When the GCA/SCA ratio is closer to one, it shows that additive gene action is largely involved in the inheritance of the trait and it will be well transmitted from the parents to the progenies. Whereas, a GCA/SCA ratio closer to zero shows that non-additive gene action is predominant in the inheritance of the trait. Narrow-sense heritability (hN2) was computed according to Eq 2 [40].

hN2=σA2σA2+σD2+σE2r (2)

where σE2 and r are the error variance and number of replications, respectively. The genotypic correlation coefficients between traits were calculated according to the formula proposed by Holland [41]. The statistical analysis was carried out using SAS [42] software.

Results

Combined analysis of variance of traits

Combined analysis of variance demonstrated that there were significant effects of different irrigation regimes on all of the traits in both the F1 hybrids and the F2 populations (S2 Table). Genetic differences between F1 hybrids and between F2 populations were highly significant for all of the studied traits. These results indicate that parents for diallel crosses had been properly selected. Also, genotype × irrigation regime interaction effects were significant for all traits in both F1 hybrids and F2 populations (S2 Table). The GCA and SCA effects were significant for all traits. Also, GCA × irrigation regime interaction effect was significant for all the traits in both the F1 and F2 generations. The SCA × irrigation regime interaction effect was significant for all traits in both the F1 and F2 generations except for TFW trait.

Effect of water-deficit stress on measured traits

DTF, DTEOF, DTR, UNPP, FUNPP, FNPP, TFW, FAC and TLY were significantly reduced under MWDS and SWDS irrigation regimes compared to the WW irrigation regime (S3 Table).

Nature of gene action

The GCA and SCA variances were highly significant for all traits in both the F1 and F2 generations (Tables 1 and 2). The GCA/SCA ratio values were high for phenological traits and relatively high for yield components in all irrigation regimes (Tables 1 and 2). These results indicate that additive gene action was predominant in controlling these traits. For FAC non-additive gene action was predominant in the F1 generation, while additive gene effects were important in the F2 generation under all irrigation regimes (Tables 1 and 2). In addition, in WW condition, non-additive gene action was predominant for TLY in both the F1 and F2 generations (Tables 1 and 2). Under MWDS and SWDS irrigation regimes, non-additive gene action was predominant for TLY in the F1 generation, while additive gene effects were important in the F2 generation (Tables 1 and 2).

Table 1. Analysis of variance for combining ability, variance components, heritability and GCA/SCA ratio estimates in the F1 generation under different irrigation regimes.

IR E DTF DTEOF DTR UNPP FUNPP FNPP TFW FAC TLY
WW GCA 1403.63** 1198.78** 753.81** 1077.65** 3741.60** 733667.03** 19.11** 59.34** 16.25**
SCA 24.55** 23.32** 6.90** 186.70** 341.13** 69891.17** 2.28** 28.44** 26.53**
Error 2.58 0.95 0.77 40.78 34.09 13083.67 0.25 2.33 0.08
σg2 57.46** 48.98** 31.12** 37.12** 141.69** 27657.33** 0.70** 1.29ns 0.03ns
σs2 7.32** 7.46** 2.04** 48.64** 102.35** 18935.83** 0.67** 8.70** 0.64**
hN2 0.93 0.93 0.96 0.54 0.71 0.70 0.65 0.19 0.08
GCA/SCA 0.94 0.93 0.97 0.60 0.73 0.74 0.68 0.23 0.09
MWDS GCA 1585.53** 1169.13** 863.31** 340.37** 40.08** 214715.62** 53.68** 101.93** 2.873**
SCA 69.52** 73.47** 59.62** 53.11** 4.09* 26472.49** 2.59** 23.03** 0.791**
Error 1.27 0.86 1.45 13.77 2.05 1120.88 0.50 1.68 0.049
σg2 63.17** 45.65** 33.49** 11.97** 1.50** 7843.46** 2.13** 3.29* 0.006*
σs2 22.75** 24.20** 19.39** 13.11** 0.68* 8450.54** 0.70** 7.12** 0.009**
hN2 0.85 0.79 0.77 0.57 0.69 0.64 0.83 0.43 0.17
GCA/SCA 0.85 0.79 0.78 0.65 0.82 0.65 0.86 0.48 0.41
SWDS GCA 1952.31** 844.83** 558.82** 64.48** 30.06** 173857.19** 47.58** 76.03** 0.68**
SCA 73.74** 42.26** 46.20** 16.38* 6.85** 19795.43** 3.08** 22.73** 0.20**
Error 1.64 1.09 0.97 7.52 2.03 2354.24 0.20 1.94 0.03
σg2 78.27** 33.44** 21.36** 2.00* 0.97** 6419.24** 1.85** 2.22* 0.02*
σs2 24.03** 13.72** 15.08** 2.95* 1.61** 5813.73** 0.96** 6.93** 0.06**
hN2 0.86 0.83 0.74 0.42 0.46 0.66 0.78 0.33 0.31
GCA/SCA 0.87 0.83 0.74 0.58 0.55 0.69 0.79 0.39 0.41

**, * and ns indicate significance at the 1% and 5% level of probability and not significant, respectively. General combining ability (GCA), specific combining ability (SCA), variance of general (σg2) and specific (σs2) combining ability, narrow-sense heritability (hN2), GCA/SCA ratio, irrigation regime (IR), estimates (E), well-watered (WW), mild water-deficit stress (MWDS), severe water-deficit stress (SWDS), days to flowering (DTF), days to the end of flowering (DTEOF), days to ripening (DTR), umbel number per plant (UNPP), fertile umbel number per plant (FUNPP), fruit number per plant (FNPP), thousand fruit weight (TFW), fatty acid content (FAC), total lipid yield (TLY).

Table 2. Analysis of variance for combining ability, variance components, heritability and GCA/SCA ratio estimates in the F2 generation under different irrigation regimes.

IR E DTF DTEOF DTR UNPP FUNPP FNPP TFW FAC TLY
WW GCA 1567.91** 1308.28** 924.18** 981.17** 3539.76** 543433.19** 15.66** 30.62** 8.30**
SCA 45.67** 40.58** 7.14** 128.32** 255.71** 29702.02* 1.47** 6.88** 6.08**
Error 5.40 1.23 0.79 35.17 28.23 13783.29 0.26 2.19 0.05
σg2 63.43** 52.82** 38.21** 35.54** 136.84** 21405.47** 0.59** 0.99** 0.004ns
σs2 13.42** 13.12** 2.12** 31.05** 75.83** 5306.24* 0.40** 1.56** 0.08**
hN2 0.87 0.86 0.96 0.59 0.72 0.80 0.66 0.35 0.05
GCA/SCA 0.90 0.89 0.97 0.70 0.78 0.89 0.75 0.56 0.10
MWDS GCA 1616.58** 1258.57** 842.25** 186.85** 34.39** 184042.28** 45.15** 119.15** 2.147**
SCA 79.20** 83.79** 62.88** 67.34* 3.37** 14090.57** 1.60** 16.00** 0.448**
Error 1.35 1.00 1.55 41.28 1.28 1790.59 0.49 1.70 0.071
σg2 64.06** 48.95** 32.47** 4.98* 1.29** 7081.32** 1.81** 4.30** 0.003**
σs2 25.95** 27.60** 20.44** 8.69* 0.70** 4099.99** 0.37** 4.77** 0.003**
hN2 0.79 0.73 0.70 0.31 0.67 0.71 0.86 0.53 0.09
GCA/SCA 0.83 0.78 0.76 0.53 0.79 0.78 0.91 0.64 0.53
SWDS GCA 1897.50** 928.53** 451.62** 53.19** 20.67** 153701.14** 40.89** 59.48** 0.48**
SCA 77.60** 52.98** 24.60** 9.59** 3.75** 15254.07** 2.04** 11.56** 0.12**
Error 2.49 1.70 2.85 3.32 0.71 1811.94 0.23 2.37 0.03
σg2 75.83** 36.48** 17.79** 1.82** 0.71** 5768.63** 1.62** 2.00** 0.02**
σs2 25.03** 17.09** 7.25** 2.09** 1.01** 4480.71** 0.61** 3.06** 0.03**
hN2 0.82 0.76 0.77 0.50 0.48 0.64 0.79 0.41 0.39
GCA/SCA 0.86 0.81 0.83 0.63 0.58 0.72 0.84 0.57 0.57

**, * and ns indicate significance at the 1% and 5% level of probability and not significant, respectively. General combining ability (GCA), specific combining ability (SCA), variance of general (σg2) and specific (σs2) combining ability, narrow-sense heritability (hN2), GCA/SCA ratio, irrigation regime (IR), estimates (E), well-watered (WW), mild water-deficit stress (MWDS), severe water-deficit stress (SWDS), days to flowering (DTF), days to the end of flowering (DTEOF), days to ripening (DTR), umbel number per plant (UNPP), fertile umbel number per plant (FUNPP), fruit number per plant (FNPP), thousand fruit weight (TFW), fatty acid content (FAC), total lipid yield (TLY).

Narrow-sense heritability

Heritability estimates for all traits are presented in Tables 1 and 2. Under WW conditions, narrow-sense heritability estimates covered a wide range of values among the different traits. They were highest for DTR where they were 0.96 in both the F1 and F2 generations. Hereditability estimates were lowest for TLY; they were 0.08 and 0.05 in the F1 and F2 generations, respectively. In MWDS, narrow-sense heritability of traits ranged from 0.17 for TLY to 0.85 for DTF in the F1 generation and 0.09 for TLY to 0.86 for DTF in F2 generation. Also under SWDS, narrow-sense heritability estimates ranged from 0.31 to 0.86 in F1 generation and from 0.39 to 0.82 in the F2 generation for TLY and DTF, respectively. Moderate to high values of narrow-sense heritability were observed for DTF, DTEOF, DTR, UNPP, FUNPP, FNPP, TFW and FAC traits under all irrigation regimes. Whereas, low values of narrow-sense heritability were obtained for TLY under all irrigation regimes (Tables 1 and 2).

Genetic combining ability analysis

GCA values of parents in both the F1 and F2 generations showed that the P6 parent was the best general combiner for phenological traits that enable plants to reach early ripening in all irrigation regimes; it had the largest negative GCA value for days to flowering (Table 3). In the case of UNPP, FUNPP and FNPP traits, the P4 parent was the best general combiner in both the F1 and F2 generations in all irrigation regimes (Table 3). Also, the P6 appeared as the best general combiner for TFW and FAC in all irrigation regimes in both the F1 and F2 generations. In the case of TLY, the P4 parent was the best general combiner in WW conditions in both the F1 and F2 generations, while in MWDS and SWDS irrigation regimes, the P6 parent had the largest positive GCA value for TLY in both the F1 and F2 generations (Table 3).

Table 3. General combining ability-effects of parents in the F1 and F2 generations under different irrigation regimes.

F1 generation
IR P DTF DTEOF DTR UNPP FUNPP FNPP TFW FAC TLY
WW P1 2.44** 3.25** 1.44** 3.77** 6.37** 209.22** 0.41** 0.32ns -0.17**
P2 5.15** 3.58** 3.78** 4.49** -8.45** -15.01ns -0.85** -1.01** 0.21**
P3 1.99** 1.58** 0.99** -2.11ns -7.57** -160.43** -0.78** -1.64** -0.20**
P4 0.78* 2.29** 1.40** 7.28** 22.78** 223.24** -0.12ns -0.60* 0.31**
P5 4.86** 3.63** 3.57** -2.10ns -4.93** -139.64** -0.22* 0.07ns 0.06ns
P6 -15.22** -14.33** -11.18** -11.33** -8.20** -117.37** 1.56** 2.86** -0.21**
MWDS P1 4.06** 3.86** 3.24** 1.58ns -0.18ns -37.68** -0.73** -0.69* -0.19**
P2 5.01** 4.78** 4.07** -2.57ns -0.43ns -82.90** -0.78** -1.74** -0.28**
P3 3.39** 3.15** 2.15** -3.62* -0.91** 2.28ns -0.61** -1.19** -0.17**
P4 -0.69** -1.97** -1.81** 4.79** 1.82** 184.93** -0.01ns -0.44ns 0.35**
P5 4.31** 3.57** 3.78** 0.86ns -1.55** -30.42** -0.86** 0.06ns -0.24**
P6 -16.07** -13.39** -11.43** -1.04ns 1.26** -36.20** 2.99** 4.01** 0.53**
SWDS P1 3.69** 2.54** 1.79** 2.80** -0.51ns -6.29ns -0.71** 0.61* -0.06ns
P2 5.53** 4.21** 4.00** -1.15* -0.98** -73.71** -0.82** -1.35** -0.14**
P3 2.69** 1.79** 1.33** -1.41** -0.82** -22.13* -0.65** -0.97** -0.06ns
P4 1.86** -0.04ns -0.29ns 1.12* 1.52** 160.93** -0.29** -0.18ns 0.11**
P5 4.44** 3.25** 2.58** -0.47ns -0.53ns -65.67** -0.38** -1.39** -0.14**
P6 -18.22** -11.75** -9.42** -0.89ns 1.33** 6.86ns 2.84** 3.28** 0.29**
F2 generation
WW P1 2.53** 3.33** 1.83** 4.01** 5.63** 163.57** 0.38** 0.05ns -0.14**
P2 5.57** 3.67** 4.17** 3.79** -6.70** -28.85ns -0.77** -0.66* 0.12*
P3 2.94** 1.88** 0.83** -2.10ns -8.51** -128.69** -0.65** -1.13** -0.10*
P4 0.90ns 2.46** 1.63** 6.55** 22.32** 212.54** -0.06ns -0.58* 0.16**
P5 4.24** 3.67** 3.92** -1.05ns -4.23** -107.83** -0.32** 0.27ns 0.06ns
P6 -16.18** -15.00** -12.38** -11.20** -8.51** -110.74** 1.41** 2.06** -0.09ns
MWDS P1 4.29** 4.00** 3.17** 2.15ns -0.27ns -13.27ns -0.64** -0.52* -0.13*
P2 5.08** 4.54** 3.63** -2.28ns -0.51* -75.36** -0.75** -2.26** -0.26**
P3 3.33** 3.13** 2.25** -2.53ns -0.84** 7.26ns -0.62** -0.98** -0.14*
P4 -0.58* -1.63** -1.58** 4.48** 1.71** 169.07** 0.06ns -0.32ns 0.33**
P5 4.13** 4.00** 3.92** -0.22ns -1.31** -42.63** -0.78** -0.21ns -0.23**
P6 -16.25** -14.04** -11.38** -1.60ns 1.21** -45.07** 2.73** 4.28** 0.43**
SWDS P1 4.13** 3.13** 1.71** 2.52** -0.27ns -9.06ns -0.51** 0.62* -0.05 ns
P2 5.21** 4.33** 3.92** -1.28** -0.96** -68.59** -0.73** -1.23** -0.11**
P3 2.71** 1.79** 0.75* -1.01** -0.62** -18.53* -0.53** -0.89** -0.06ns
P4 1.96** -0.21ns -0.25ns 1.04** 1.20** 152.83** -0.39** -0.22ns 0.09*
P5 4.00** 3.25** 2.25** -0.31ns -0.47** -59.39** -0.50** -1.16** -0.11**
P6 -18.00** -12.29** -8.38** -0.95** 1.12** 2.74ns 2.66** 2.88** 0.25**

**, * and ns indicate significance at the 1% and 5% level of probability and not significant, respectively. Irrigation regime (IR), parents (P), well-watered (WW), mild water-deficit stress (MWDS), severe water-deficit stress (SWDS), days to flowering (DTF), days to the end of flowering (DTEOF), days to ripening (DTR), umbel number per plant (UNPP), fertile umbel number per plant (FUNPP), fruit number per plant (FNPP), thousand fruit weight (TFW), fatty acid content (FAC), total lipid yield (TLY).

Results of SCA analysis for DTF, DTEOF and DTR indicated that the progenies of P6 (H1×6, H2×6, H3×6, H4×6 and H5×6) displayed negative significant SCA-effects in both the F1 and F2 generations in all irrigation regimes (Tables 4 and 5). In WW conditions, the crosses of H1×6 and H4×6 had the largest positive significant SCA values for UNPP in both the F1 and F2 generations. Under MWDS conditions, the SCA value for UNPP was not significant. Under SWDS conditions, the cross of H1×6 had the largest positive significant SCA value for UNPP in the F1 generation, whereas, in the F2 generation, the population of H3×6 had the largest positive significant SCA value for this trait (Tables 4 and 5).

Table 4. Specific combining ability-effects in the F1 generation under different irrigation regimes.

IR F1s DTF DTEOF DTR UNPP FUNPP FNPP TFW FAC TLY
WW H1×2 1.39ns 1.64** 0.19ns 7.61* 1.21ns 172.84** -0.39ns 1.77** -0.80**
H1×3 1.22ns -0.02ns 0.65ns -6.26ns 5.06ns 60.59ns 0.48ns -0.93ns 0.63**
H1×4 0.10ns 1.27* -0.43ns 2.39ns 9.59** -51.48ns 0.20ns 4.69** 1.18**
H1×5 0.68ns 0.93ns 1.07ns -0.03ns -2.41ns 76.86ns -0.17ns -2.64** 0.09ns
H1×6 -4.90** -5.11** -1.18* 13.29** 5.89ns 200.96** 1.02** 2.90** 0.31ns
H2×3 0.51ns -0.02ns -0.68ns 2.82ns -5.35ns -16.84ns -0.41ns 0.07ns 0.29ns
H2×4 1.05ns 1.27* -0.10ns -1.13ns -5.93ns -13.15ns -0.77** 0.02ns 1.01**
H2×5 -0.36ns -0.40ns 1.07ns 0.11ns -7.26** 61.73ns 0.62* 3.36** 0.44*
H2×6 -2.61** -3.11** 0.48ns -6.50ns -3.22ns -110.44ns 1.14** 0.57ns 0.61**
H3×4 -0.11ns 0.27ns 2.69** 6.37ns 16.02** 99.81ns -0.24ns -2.02* 0.01ns
H3×5 0.80ns 0.60ns 1.52** -0.69ns -6.91* -277.58** -0.25ns 1.65* -0.20ns
H3×6 -2.45** -1.44* 0.94ns -1.03ns -9.47** -6.26ns 0.97** 3.19** 0.50**
H4×5 1.68* 0.56ns -0.56ns 0.99ns 15.05** 217.25** 0.19ns 2.27** 0.66**
H4×6 -2.24** -3.15** 1.86** 15.49** 9.19** 117.04* 0.58* 3.15** 0.04ns
H5×6 -3.32** -2.15** -2.31** -5.64ns -4.71ns -52.25ns 0.69** -1.18ns 0.41*
MWDS H1×2 1.76** 2.49** 1.25* 1.40ns -0.90ns -113.00** -0.08ns 3.83** -0.06ns
H1×3 2.05** 3.78** 2.17** -4.85ns -0.95ns -179.89** -0.44ns -1.71* -0.19ns
H1×4 -0.20ns -2.10** -1.88** -2.96ns 0.01ns 89.33** -0.76* -0.13ns 0.46**
H1×5 3.13** -0.30ns 1.88** -4.93ns -0.12ns -17.55ns -0.10ns -0.63ns -0.20ns
H1×6 -6.16** -3.35** -4.25** 4.64ns 2.01** 97.23** 1.25** 3.08** 0.41**
H2×3 4.09** 4.20** 2.33** -6.62ns -0.87ns -41.18* -0.24ns -0.01ns -0.05ns
H2×4 -2.16** -4.68** -4.38** 1.47ns 0.76ns 106.02** -0.06ns 0.24ns 0.39**
H2×5 0.51ns 4.78** 3.38** -4.77ns 0.10ns -17.13ns -0.32ns 5.08** -0.01ns
H2×6 -6.45** -5.93** -3.42** 5.07ns 0.62ns 77.30** 1.16** -2.21** 0.19ns
H3×4 -3.20** -4.72** -2.13** -0.12ns 0.01ns 11.07ns 0.40ns -0.96ns 0.01ns
H3×5 2.80** 2.07** 2.96** 0.14ns -0.38ns -38.61* -0.08ns 1.54* 0.00ns
H3×6 -3.83** -4.30** -3.17** 5.95ns 0.77ns 62.35** 0.88* 2.91** 0.54**
H4×5 -2.45** -4.14** -7.08** -1.50ns 1.38ns 37.44* -0.01ns 1.12ns 0.55**
H4×6 -1.74** -0.51ns -0.21ns 2.57ns 0.67ns -120.31** 0.89* 2.49** 0.74**
H5×6 -5.08** -4.39** -4.46** 5.90ns 0.78ns 64.44** 0.88* -3.67** 0.01ns
SWDS H1×2 3.24** 1.49** 1.68** -0.20ns -0.20ns 4.65ns -0.46* 1.91** -0.07ns
H1×3 2.74** 3.57** 1.35* -1.08ns -0.25ns 10.32ns 0.01ns -0.13ns -0.12ns
H1×4 1.24ns -0.93ns 2.31** 2.73ns 0.27ns 73.51** 0.24ns 3.08** 0.13ns
H1×5 3.32** 4.11** 4.10** -3.28* -0.71ns -57.45* -0.17ns -2.05** -0.17ns
H1×6 -6.68** -2.55** -3.23** 3.07* 1.69* 50.02ns 0.64** 1.95** 0.43**
H2×3 2.90** 4.24** 4.14** -0.36ns -0.55ns -20.22ns -0.75** -1.17ns 0.06ns
H2×4 0.74ns -0.60ns 0.43ns 0.35ns 1.31ns 86.91** 0.12ns 0.70ns 0.18ns
H2×5 3.15** 1.78** 3.89** 1.47ns -0.34ns -35.03ns 0.34ns 2.91** -0.11ns
H2×6 -5.51** -5.89** -3.77** 0.59ns 1.23ns 67.52** 1.65** 3.24** 0.12ns
H3×4 0.90ns 0.15ns 3.10** 0.67ns 0.88ns 57.22* 0.15ns -1.01ns 0.10ns
H3×5 1.65* -0.80ns 3.56** -0.73ns -0.47ns 5.79ns -0.16ns 1.87** 0.01ns
H3×6 -3.68** -2.47** -1.77** 1.62ns 1.40ns 43.43ns 0.79** 2.20** 0.21ns
H4×5 2.15** 0.70ns -1.15* -0.33ns 1.33ns 105.41** -0.06ns 1.74* 0.25*
H4×6 -3.85** -1.30* -0.82ns 2.42ns 1.40ns -4.69ns 1.22** 2.41** 0.18ns
H5×6 -5.43** -4.60** -3.69** 2.35ns 1.05ns 88.15** 0.98** -2.38** 0.19ns

**, * and ns indicate significance at the 1% and 5% level of probability and not significant, respectively. Irrigation regime (IR), well-watered (WW), mild water-deficit stress (MWDS), severe water-deficit stress (SWDS), days to flowering (DTF), days to the end of flowering (DTEOF), days to ripening (DTR), umbel number per plant (UNPP), fertile umbel number per plant (FUNPP), fruit number per plant (FNPP), thousand fruit weight (TFW), fatty acid content (FAC), total lipid yield (TLY).

Table 5. Specific combining ability-effects of the F2 generation under different irrigation regimes.

IR F1s DTF DTEOF DTR UNPP FUNPP FNPP TFW FAC TLY
WW H1×2 -1.40ns 1.95** 1.33** 2.69ns 3.48ns 59.66ns -0.43ns 1.37ns -0.49**
H1×3 0.89ns 0.74ns 0.00ns -0.51ns 2.96ns 47.42ns 0.46ns -0.56ns 0.19ns
H1×4 0.27ns -0.17ns 0.54ns 0.28ns 6.76* -26.76ns 0.23ns 1.96** 0.42**
H1×5 0.60ns 0.62ns 0.25ns -0.45ns 0.13ns 38.12ns -0.46ns -1.86* -0.17ns
H1×6 -2.32* -6.38** -1.46** 14.42** 3.93ns 135.93* 0.63* -0.66ns 0.25ns
H2×3 2.85* -1.26ns 1.00* 2.25ns -5.30ns -8.04ns -0.03ns -0.28ns -0.07ns
H2×4 0.56ns 0.83ns 0.21ns 2.47ns 1.03ns -21.12ns -0.78** 0.12ns 0.32*
H2×5 2.23ns -0.38ns -0.42ns -1.63ns -5.40ns 70.52ns 0.25ns 1.65* -0.08ns
H2×6 -3.36** -3.71** -0.79ns -7.24* -6.50* -85.05ns 0.90** -0.15ns 0.15ns
H3×4 2.85* 0.62ns 2.21** 4.27ns 10.63** 77.09ns -0.61* -1.70* -0.16ns
H3×5 -0.48ns 0.41ns 1.58** -3.86ns -4.46ns -122.20* -0.41ns 1.17ns 0.03ns
H3×6 -3.07** -2.26** -1.46** -2.44ns -7.42** -30.39ns 1.07** 0.86ns 0.28*
H4×5 0.89ns 0.83ns 1.13* 3.06ns 12.18** 143.20* 0.41ns 0.63ns 0.17ns
H4×6 -4.36** -3.51** -0.92ns 9.57** 12.34** 131.48* 0.36ns 2.71** 0.22ns
H5×6 -7.02** -4.05** -1.54** 0.26ns -5.06ns 0.81ns 0.27ns -1.81* 0.13ns
MWDS H1×2 1.24* 2.60** 1.40* -2.12ns -0.61ns 8.69ns -0.03ns 1.74* -0.05ns
H1×3 2.66** 3.35** 1.44* -3.96ns -1.20* -85.61** -0.09ns -1.58* -0.16ns
H1×4 0.24ns -2.57** -2.06** 1.04ns 0.10ns 45.52* -0.47ns -0.67ns 0.30ns
H1×5 1.87** 1.14* 1.11ns -2.70ns 0.16ns -60.84** -0.39ns -0.46ns -0.14ns
H1×6 -5.42** -4.15** -4.27** 4.39ns 1.02ns 68.71** 1.01* 4.45** 0.40**
H2×3 3.54** 3.81** 2.32** -0.26ns -0.64ns -59.14** -0.28ns 0.51ns 0.03ns
H2×4 -2.88** -4.77** -5.85** -1.65ns 0.42ns 67.96** 0.35ns 0.32ns 0.15ns
H2×5 2.41** 4.27** 2.32** -2.92ns -0.36ns -24.65ns -0.42ns 3.58** -0.04ns
H2×6 -7.21** -6.69** -4.06** 5.75ns 0.37ns 52.02* 0.77* -2.98** 0.06ns
H3×4 -2.46** -4.36** -1.81** 1.42ns -0.17ns -20.99ns 0.00ns -0.79ns 0.01ns
H3×5 2.49** 2.68** 3.02** -3.55ns 0.16ns -63.27** -0.12ns 0.97ns 0.02ns
H3×6 -5.46** -5.27** -3.68** 6.28ns 0.49ns 65.35** 0.78* 1.82** 0.35*
H4×5 -3.92** -3.57** -6.14** -3.65ns 0.90ns 69.70** 0.40ns 0.32ns 0.23ns
H4×6 -1.21* -0.19ns -0.18ns 2.16ns 0.95ns -99.36** 0.26ns 2.41** 0.79**
H5×6 -5.59** -5.48** -4.35** 4.41ns 1.63** 61.51** 0.99** -3.70** -0.03ns
SWDS H1×2 3.24** 2.88** 1.52ns -2.04* -0.11ns -6.64ns -0.34ns 1.68* -0.09ns
H1×3 1.74* 3.08** 1.68ns -1.29ns -0.04ns -6.52ns 0.08ns -0.01ns -0.08ns
H1×4 4.49** 2.75** 2.35** 0.97ns 0.30ns 46.00* -0.01ns 2.11** 0.10ns
H1×5 1.11ns 2.96** 2.52** -1.76ns -0.62ns -22.45ns 0.04ns -1.85* -0.14ns
H1×6 -7.22** -3.83** -3.52** 1.45ns 1.30** 45.98* 1.15** 0.84ns 0.12ns
H2×3 2.65** 4.54** 3.14** -1.61ns -0.15ns -0.53ns 0.08ns -0.85ns 0.06ns
H2×4 0.40ns -0.13ns 0.48ns 0.78ns 1.01* 84.85** 0.37ns 1.01ns 0.17ns
H2×5 0.36ns 0.75ns -0.02ns -0.16ns -1.09* -29.80ns 0.05ns 1.85* -0.11ns
H2×6 -5.64** -6.71** -0.40ns 1.55ns 0.96* 62.85** 0.95** 2.35** 0.14ns
H3×4 -1.43ns -1.92** 0.64ns 1.38ns 0.64ns 59.64** -0.20ns -1.52ns 0.04ns
H3×5 4.20** 1.96** 3.48** -1.52ns -0.38ns 3.06ns -0.20ns 1.77* 0.03ns
H3×6 -4.80** -3.17** -2.23** 1.99* 0.86ns 41.73ns 0.62** 0.69ns 0.10ns
H4×5 -0.05ns -1.38* 0.81ns -0.82ns 0.73ns 92.83** -0.16ns 1.20ns 0.21ns
H4×6 -4.05** -2.17** -1.57ns 0.42ns 0.34ns -10.94ns 1.12** 1.94* 0.33**
H5×6 -4.76** -3.29** -2.73** 1.64ns 1.55** 74.78** 0.53* -2.00* 0.15ns

**, * and ns indicate significance at the 1% and 5% level of probability and not significant, respectively. Irrigation regime (IR), well-watered (WW), mild water-deficit stress (MWDS), severe water-deficit stress (SWDS), days to flowering (DTF), days to end the of flowering (DTEOF), days to ripening (DTR), umbel number per plant (UNPP), fertile umbel number per plant (FUNPP), fruit number per plant (FNPP), thousand fruit weight (TFW), fatty acid content (FAC), total lipid yield (TLY).

Under WW conditions, the progenies of the P4 parent (H1×4, H3×4, H4×5 and H4×6) had the largest positive significant SCA values for FUNPP in both the F1 and F2 generations. In MWDS and SWDS conditions, the crosses of H1×6 and H5×6 had the largest positive significant SCA values for FUNPP in both the F1 and F2 generations (Tables 4 and 5).

In case of FNPP, the cross of H4×5 had the largest positive significant SCA value in WW and SWDS irrigation regimes in both the F1 and F2 generations. In MWDS conditions, the crosses of H2×4 and H4×5 had the positive significant SCA values for FNPP in both the F1 and F2 generations. For TFW, the progenies of the P6 parent (H1×6, H2×6, H3×6, H4×6 and H5×6) had positive significant SCA values in all irrigation regimes in both the F1 and F2 generations (Tables 4 and 5).

Under WW conditions, the crosses of H1×4, H1×6, H3×6, H4×5 and H4×6 had positive significant SCA values for FAC in the F1 generation whereas, in the F2 generation, the populations of H1×4 and H4×6 had the positive significant SCA values. In MWDS conditions, the crosses of H1×6, H3×6 and H4×6 had the positive significant SCA values for FAC in both the F1 and F2 generations. Also, in SWDS conditions, the crosses of H1×6 and H4×5 had a positive significant SCA value for FAC in the F1 generation and the population of H4×6 had a positive significant SCA value in the F2 generation (Tables 4 and 5).

Under WW conditions, the crosses of H1×4 and H2×4 had the largest positive significant SCA values for TLY in both the F1 and F2 generations. Under MWDS conditions, the crosses of H1×6, H3×6 and H4×6 had the largest positive significant SCA values for TLY in both the F1 and F2 generations. Also, under SWDS conditions, the crosses of H1×6 and H4×5 had the largest positive significant SCA values for TLY in the F1 generation and the population of H4×6 had the largest positive significant SCA value in the F2 generation (Tables 4 and 5).

Genetic correlation of total lipid yield with phenological and morphological traits

Genetic correlation analysis under WW conditions showed that there were positive correlations between total lipid yield and all of the phenological and yield components traits (S4 Table). In MWDS and SWDS conditions, total lipid yield was significantly and positively correlated with yield components while total lipid yield had the significant negative correlation with phenological traits (S4 Table).

Discussion

Iran has special a geographical location with high genetic diversity for coriander, as well as many other crops. It is a promising place to find and gather new genetic resources for coriander anda high genetic diversity was previously reported for drought stress tolerance for Iranian coriander genotypes [35]. Using drought-tolerant and high-yielding coriander genotypes as the parents in crossing programs can significantly increase the efficiency of coriander breeding schemes for developing high-yielding coriander genotypes for arid and semi-arid areas. In this study, a large genetic variation was observed for phonological and yield components, total lipid yield and fatty acid content among the parental genotypes, F1 hybrids and F2 populations. This indicates the existence of an excellent potential to study coriander genetics relative to crop improvement.

Results showed that flowering and maturity times were decreased in MWDS and SWDS irrigation regimes. In agreement with our results, Gales and Wilson [43] with studies in winter wheat, Bannayan et al. [20] in isabgol and black cumin and Alinian and Razmjoo [23] in cumin (Cuminum Cyminum L.) reported that water-deficit stress induced a reduction in the time to maturity. This effect is influenced by various factors including the level and duration of the stress, the genotype and the maturity time under non-stress conditions. Reduced time to maturity is known as a water-deficit stress avoidance mechanism in plants [43].

Yield components’ measurements were higher under WW condition than with MWDS and SWDS irrigation regimes. Reduction in yield components may be due to lower availability of nutrients along with reduced photosynthesis and reduced translocation of photosynthesis products from source to sink area under drought stress [44]. The preferential allocation of biomass to the root growth has been associated with yield reduction in coriander under water stress conditions [26]. Similarly, Alinian and Razmjoo [23] reported that number of umbels per plant, number of seeds per umbel and 1000 seeds weight were reduced under water-deficit stress in cumin accessions.

The highest fatty acid content and total lipid yield values observed under WW conditions, conversely the lowest fatty acid content and total lipid yield values were obtained in SWDS conditions in both the F1 hybrids and the F2 populations. Similar results were reported by Singh and Ramesh [45] in rosemary, Zehtab-Salmasi et al. [19] in dill (Anethum graveolens L.), Hamrouni et al. [13] in safflower, Bettaieb et al. [11] in Salvia officinalis L. and Bettaieb et al. [14] in cumin (Cuminum cyminum L.). Those studies observed that fatty acid content and total lipid yield were significantly decreased by water-deficit stress. Reduction in total lipid yield under water-deficit stress could also be due to the reduction in days to flowering and maturity and some of the yield components for plants.

Narrow-sense heritability and GCA/SCA ratio values suggest that additive genetic effects were predominant in controlling phenological and yield components traits in all irrigation regimes in both the F1 and F2 generations. Therefore, breeding methods based on selection can be effective in the F2 generations for improvement of these traits. Similar to our findings, Amiri-Oghan et al. [25] observed a high heritability for days to flowering and days to maturity in oilseed rape (Brasica napus L.). FAC and TLY In coriander were predominantly affected by non-additive gene action in the F1 generation while additive gene action predominated in the F2 generation under MWDS and SWDS conditions. Therefore, breeding methods based on selection in the F2 and later generations will likely be effective to improve FAC and TLY in MWDS and SWDS conditions. The results of narrow-sense heritability and the GCA/SCA ratio for TLY in WW indicate that non-additive type of gene action were predominant in both the F1 and F2 generations. Therefore, for improvement of TLY under WW conditions, selection should be deferred to the later generations of segregation in which non-additive genetic effects have been reduced or fixed.

Blum [46] reported that indirect selection for yield components and other traits that have high heritability and which are strongly correlated with economical yield could be more efficient than direct selection for yield. In this study phenological and TFW traits had higher heritability estimates than total lipid yield. These traits also had a significant genetic correlation with total lipid yield. Thus, selection for DTF, DTEOF, DTR and TFW traits may be effective criteria for improvement of total lipid yield, especially under water stress conditions. The significant and negative genetic correlations between total lipid yield and phenological traits in MWDS and SWDS conditions suggest that simultaneous improvement of earliness to cope with drought stress and total lipid yield can be achieved in coriander. Flowering is the most critical stage that influences the yield of coriander. The time of flower initiation can have a strong influence on the number of flowers, umbel number per plant, fertile umbel number per plant and fruit number per plant. The development of early ripening coriander genotypes is important to avoid abiotic stresses, particularly drought and high temperatures at the end of the growing season in arid and semi-arid environments. Such conditions have been observed in some areas of Iran and coriander production has been restricted by the adverse effects of the terminal heat and drought stress which cause a reduction in the number of successfully pollinated flowers. Overall, the importance of phenological traits and the use of early genotypes as donor parents should be considered in coriander breeding programs to improve total lipid yield.

Achievement in breeding programs depends on the careful choice of parents. The selection of parents for hybridization programs should be based on their genetic value. High GCA values of the parents are mainly due to the additive genetic effects [37] that are heritable in the segregating generations. Therefore, the selection of parents for hybridization should be based on their GCA-effects which reflect on their potential to produce superior segregates in the F2 and later generations. The GCA-effects of the six parents on the traits measured in both the F1 and F2 generations showed that the P4 appeared as the best general combiner for UNPP, FUNPP and FNPP. P6 was the best general combiner for DTF, DTEOF, DTR, TFW, FAC and TLY. These parents could be used to develop early flowering and early maturing types coupled with high total lipid yield genotypes in advanced segregating generations. The offspring of the P4 and P6 parents had high SCA values for FAC and TLY. They also exhibited significant SCA values for other phenological and yield components traits in both F1 and F2 generations. Results of the GCA and SCA analysis indicate that many of crosses which showed significant SCA-effects also had high GCA values for all traits.

Conclusion

Large genetic variability for phenological, yield components, total lipid yield and fatty acid content indicate a high potential of the studied germplasm for genetic improvement in coriander. The results indicated that water-deficit stress decreased DTF, DTEOF, DTR, UNPP, FUNPP, FNPP, TFW, FAC and TLY in both F1 hybrids and F2 generations. The high narrow-sense heritability and GCA/SCA ratio for phenological traits indicates that these traits are mainly governed by additive genetic effects and suggest that these traits can be used as reliable and heritable selection criteria under drought stress. These traits were also correlated with total lipid yield and could be used as suitable surrogate selection criteria to enhance total lipid yield and to identify superior genotypes for drought stress conditions. Based on their general combining ability, the P4 and P6 parents can be used as promising parents for hybridization and selection of genotypes with high total lipid yield coupled with early ripening in advanced generations of segregation.

Supporting information

S1 Data File. Supporting information file for the paper, data file used in this manuscript’s analyses.

(XLS)

S1 Table. Soil properties of different layers of the experimental field.

FC, soil moisture at field capacity.

(DOC)

S2 Table. Combined analysis of variance for traits in the F1 and F2 progenies and their parents under drought environment.

**,* and ns indicate significance at the 1% and 5% level of probability and not significant, respectively. Environment (E), replication (R), genotype (G), general combining ability (GCA), specific combining ability (SCA), days to flowering (DTF), days to the end of flowering (DTEOF), days to ripening (DTR), umbel number per plant (UNPP), fertile umbel number per plant (FUNPP), fruit number per plant (FNPP), thousand fruit weight (TFW), fatty acid content (FAC), total lipid yield (TLY).

(DOC)

S3 Table. The mean of traits in coriander under different irrigation regimes in the F1 and F2 generations.

In each column, the values with the same letters do not differ significantly. Well-watered (WW), mild water-deficit stress (MWDS), severe water-deficit stress (SWDS), days to flowering (DTF), days to the end of flowering (DTEOF), days to ripening (DTR), umbel number per plant (UNPP), fertile umbel number per plant (FUNPP), fruit number per plant (FNPP), thousand fruit weight (TFW), fatty acid content (FAC), total lipid yield (TLY).

(DOC)

S4 Table. Genetic correlation coefficients and their standard error (SE) between total lipid yield and other traits under different irrigation regimes.

Well-watered (WW), mild water-deficit stress (MWDS), severe water-deficit stress (SWDS), days to flowering (DTF), days to the end of flowering (DTEOF), days to ripening (DTR), umbel number per plant (UNPP), fertile umbel number per plant (FUNPP), fruit number per plant (FNPP), thousand fruit weight (TFW), total lipid yield (TLY). ** indicates statistical significance at the 1% level of probability.

(DOC)

Acknowledgments

The authors thank from the Gene bank of the Seed and Plant Improvement Institute of Karaj, Iran for making available plant materials.

Data Availability

All relevant data are within the paper and its Supporting Information files.

Funding Statement

This work was supported by Tarbiat Modares University. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

S1 Data File. Supporting information file for the paper, data file used in this manuscript’s analyses.

(XLS)

S1 Table. Soil properties of different layers of the experimental field.

FC, soil moisture at field capacity.

(DOC)

S2 Table. Combined analysis of variance for traits in the F1 and F2 progenies and their parents under drought environment.

**,* and ns indicate significance at the 1% and 5% level of probability and not significant, respectively. Environment (E), replication (R), genotype (G), general combining ability (GCA), specific combining ability (SCA), days to flowering (DTF), days to the end of flowering (DTEOF), days to ripening (DTR), umbel number per plant (UNPP), fertile umbel number per plant (FUNPP), fruit number per plant (FNPP), thousand fruit weight (TFW), fatty acid content (FAC), total lipid yield (TLY).

(DOC)

S3 Table. The mean of traits in coriander under different irrigation regimes in the F1 and F2 generations.

In each column, the values with the same letters do not differ significantly. Well-watered (WW), mild water-deficit stress (MWDS), severe water-deficit stress (SWDS), days to flowering (DTF), days to the end of flowering (DTEOF), days to ripening (DTR), umbel number per plant (UNPP), fertile umbel number per plant (FUNPP), fruit number per plant (FNPP), thousand fruit weight (TFW), fatty acid content (FAC), total lipid yield (TLY).

(DOC)

S4 Table. Genetic correlation coefficients and their standard error (SE) between total lipid yield and other traits under different irrigation regimes.

Well-watered (WW), mild water-deficit stress (MWDS), severe water-deficit stress (SWDS), days to flowering (DTF), days to the end of flowering (DTEOF), days to ripening (DTR), umbel number per plant (UNPP), fertile umbel number per plant (FUNPP), fruit number per plant (FNPP), thousand fruit weight (TFW), total lipid yield (TLY). ** indicates statistical significance at the 1% level of probability.

(DOC)

Data Availability Statement

All relevant data are within the paper and its Supporting Information files.


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