Table 1.
Mean Values for Flow Cytometric Assessment of MPIO-Labeled Immune Cells in the Spleen and Bone Marrow
| Gate | Spleen | Bone Marrow |
||
|---|---|---|---|---|
| Lymphocyte | Mean | SD | Mean | SD |
| MPIO+ | 0.57 | 0.07 | 0.69 | 0.09 |
| T cells: CD3+ MPIO+ | 0.19 | 0.04 | 0.31 | 0.06 |
| CD3+ as % MPIO+ | 33.35 | 4.03 | 45.19 | 4.85 |
| MPIO+ | 0.53 | 0.11 | 0.41 | 0.07 |
| B cells: CD19+ MPIO+ | 0.28 | 0.09 | 0.05 | 0.05 |
| CD19+ as % MPIO+ | 51.47 | 6.76 | 12.38 | 10.08 |
| Monocyte/Neutrophil/Eosinophil | ||||
| MPIO+ | 3.00 | 0.49 | 0.50 | 0.14 |
| CD11b+MPIO+ | 1.06 | 0.16 | 0.46 | 0.13 |
| CD11b+ as % MPIO+ | 39.20 | 5.27 | 92.15 | 3.48 |
| Total CD11b+ | 31.26 | 2.51 | 84.55 | 5.02 |
Mean percentages and standard deviations (SD) obtained from the spleen and the bone marrow of 7 MPIO-injected mice are shown. In each box, MPIO+ is the total percentage of cells in the indicated gate (lymphocyte or monocyte/neutrophil/eosinophil) that were MPIO-labeled, as determined by acquisition of Dragon Green Fluorescence. The shaded line below this indicates the percentage of cells that were positive for both MPIO and the given marker. “% MPIO” is the percentage of the total MPIO+ population that also stained positive for this marker.