Table 1.
PCR primers used in this study. Expectation of amplification determined by querying the primer sequences via BLASTn to nr/nt database. HP = hypothetical protein.
| Primer Pair | Forward Primer (5′–3′) | Reverse Primer (3′–5′) | Annotated Coding Sequence (CDS) in Amplicon | Strains/Species Expected to Amplify |
|---|---|---|---|---|
| 1 | CTACGGCCGTGCAGAC | CTCCATGTGTGGCATCC | PB1 gp10 (HP), PB1 gp11 (HP) | SPM-1, PB1, F8 |
| 2 | ACCTTCTTCGGCATCCTC | TGTGGTCACCGTATTCCA | PB1 gp23 (HP), PB1 gp24 (HP) | DL60, DL52, vB_PaeM_C1-14_Ab28, SPM-1, PB1, F8 |
| 3 | CGCCATAATAGGCTCCAA | CGAGACATTCGCTGATGA | PB1 gp55 (helicase) | SPM-1, PB1, F8 |
| 4 | ACCGACTCACGACGATGG | CGGCAAGGTGTTCGCTTA | PB1 gp68 (HP), PB1 gp69 (HP) | PB1 |
| 5 | CGTCGAGGATGCTGATGG | GGCAGGTCCGAAGGCTAC | PB1 gp84 (HP), PB1 gp85 (HP), PB1 gp86 (HP) | vB_PaeM_LS1, vB_PaeM_E215, KPP22M1, vB_PaeM_E217, NP3, phiKTN6, KPP22M3, KPP22M2, KP22, vB_Pae_PS44, LMA2, vB_PaeM_CEB_DP1, vB_PaeM_PAO1_Ab29, vB_PaeM_PAO1_Ab27, KPP12, NH-4, PB1 |