Table 1.
Plasmids and primers used in this study.
| Plasmids | ||
| Description | Reference | |
| pET28a | Kanr, T7 promoter, His-tagged expression vector | Novagen, Wisconsin, SA |
| pET28a-EGFP | pET28a with EGFP | This study |
| pET28a-LysSA12 | pET28a with LysSA12 (56 kDa) | [19] |
| pET28a-LSA12CBD | pET28a with LSA12CBD | This study |
| pET28a-LSA12CHAP | pET28a with LSA12CHAP (20 kDa) | This study |
| pET28a-LSA12CHAPCBD | pET28a-LSA12CBD with LSA12CHAP (32 kDa) | This study |
| pET28a-LSA12AMICBD | pET28a with LSA12AMICBD (44 kDa) | This study |
| pET28a-LysSA97 | pET28a with LysSA97 (56 kDa) | [19] |
| pET28a-LSA97CBD | pET28a with LSA97CBD | This study |
| pET28a-LSA97CHAPCBD | pET28a-LSA97CBD with LSA97CHAP (33 kDa) | This study |
| pET28a-LSA97AMICBD | pET28a with LSA97AMICBD (38 kDa) | This study |
| pET28a-EGFP_LSA12CBD | pET28a-EGFP with LSA12CBD (42 kDa) | This study |
| pET28a-EGFP_LSA12AMICBD | pET28a-EGFP with LSA12AMICBD (66 kDa) | This study |
| pET28a-EGFP_LSA12AMI | pET28a-EGFP with LSA12AMI (54 kDa) | This study |
| pET28a-EGFP_LSA97CBD | pET28a-EGFP with LSA97CBD (42 kDa) | [7] |
| pET28a-EGFP_LSA97AMICBD | pET28a-EGFP with LSA97AMICBD (65 kDa) | This study |
| pET28a-EGFP_LSA97AMI | pET28a-EGFP with LSA97AMI (52 kDa) | This study |
| Primers (5′→3′) a | ||
| Sequence | Purpose | |
| BamH1_LSA12CHAP_F | AAA GGA TCC ATGC AAG CAA AAC TAA CTA AAA A | pET28a-LSA12CHAP and pET28a-LSA12CHAPCBD construction |
| LSA12CHAP_Sal1_R | TTT GTC GAC TGA TCG TGG AGC TGT TTC GCT T | pET28a-LSA12CHAP construction |
| LSA12CHAP_EcoR1_R | TTT GAA TTC TGA TCG TGG AGC TGT TTC GCT T | pET28a-LSA12CHAPCBD construction |
| BamH1_LSA12AMI_F | AAA GGA TCC GTA CAA TCT CCT ACG CAA GCA | pET28a-LSA12AMI, pET28a-LSA12AMICBD, pET28a-EGFP_LSA12AMI and pET28a-EGFP_LSA12AMICBD construction |
| LSA12AMI_Sal1_R | TTT GTC GAC ACT TGA AGC GCT TGA CTC ATT AG | pET28a-LSA12AMI and pET28a-EGFP_LSA12AMI construction |
| EcoR1_LSA12CBD_F | AAA GAA TTC TCA AGT AAT ACA GTT AAA CCA GT | pET28a-LSA12CBD construction |
| LSA12CBD_Sal1_R | TTT GTC GAC ACT GAT TTC TCC CCA TAA GT | pET28a-LSA12CBD, pET28a-LSA12AMICBD and pET28a-EGFP_LSA12AMICBD construction |
| BamH1_LSA97CHAP_F | AAA GGA TCC ATG CCG TCG GTT AGG ACA TAC AG | pET28a-LSA97CHAP and pET28a-LSA97CHAPCBD construction |
| LSA97CHAP_Sal1_R | TTT GTC GAC TTC TTT TGC GTA GAA TGG ACG GAT | pET28a-LSA97CHAPconstruction |
| LSA97CHAP_EcoR1_R | TTT GAA TTC TTC TTT TGC GTA GAA TGG ACG GAT | pET28a-LSA97CHAPCBD construction |
| BamH1_LSA97AMI_F | AAA GGA TCC CAA GAT AAG TTA TCA AAA GGT AAA | pET28a-LSA97AMICBD, pET28a-EGFP_LSA97AMICBD and pET28a-EGFP_LSA97AMI construction |
| LSA97AMI_Sal1_R | TTT GTC GAC ACT ACT TGG CGC ATC AAT TTG TC | pET28a-EGFP_LSA97AMI construction |
| EcoR1_LSA97CBD_F | AAA GAA TTC AGT AGT AAG CCA AGC GCT GAC AA | pET28a-LSA97CBD construction |
| LSA97CBD_Sal1_R | TTT GTC GAC TTA AGC CCA CTC AAT CGT GCC CCA | pET28a-LSA97CBD, pET28a-LSA97AMICBD and pET28a-EGFP_LSA97AMICBD construction |
| Nde1_EGFP_F | AAA CAT ATG ATG GTG AGC AAG GGC GAG GA | pET28a-EGFP construction |
| EGFP_BamH1_R | TTT GGA TCC CTT GTA CAG CTC GTC CAT GCC G | |
a Restriction sites are underlined.