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. 2018 Jun 11;145(11):dev160127. doi: 10.1242/dev.160127

Fig. 1.

Fig. 1.

CLIn reveals glial cells made by type II NBs. Representative images of anti-mCD8 immunostained (green) and nc82-counterstained (blue) adult fly brains. (A) Genetics for labeling all brain glia (repo-GAL4 driving UAS-mCD8::GFP expression). (B-C‴) Complete expression pattern of brain glia (n=5). (B) Maximum projection image of a z-stack. (C-C‴) Single confocal sections showing glia distribution from posterior (C) to anterior (C‴). MB calyx (CA), protocerebral bridge (PB), inferior bridge (IB), superior posterior slope (SPS), inferior posterior slope (IPS), lobula (LO), lobula plate (LOP), medulla (ME), superior medial procerebrum (SMP), superior intermediate protocerebrum (SIP), superior lateral protocerebrum (SLP), superior clamp (SCL), inferior clamp (ICL), pedunculus (PED), lateral horn (LH), fan-shaped body (FB), noduli (NO), gorget (GOR), posteriorlateral protocerebrum (PLP), vest (VES), wedge (WED), posterior ventrolateral protocerebrum (PVLP), anterior ventrolateral protocerebrum (AVLP), ellipsoid body (EB), MB medial and vertical lobes (ML and VL), spur (SPU), crepine (CRE), epaulette (EPA), lateral accessory lobe (LAL), anterior optic tubercle (AOTU), antennal lobe (AL), and gnathal ganglia (GNG). We follow the naming nomenclature of Ito et al. (2014). (D) CLIn technique marks only the repo-GAL4-positive glia within type II NB lineages targeted with stg14-KD. The∩represents an intersection. (E) Glia innervating only subsets of brain neuropils, particular the CX, are visible after intersection. n=7. (F-F‴) Single confocal sections showing the major neuropils outlined and covered by glia of type II lineages. (G) Independent labeling of type II glia and all brain glia via dual genetic systems (GAL4-UAS and LexAp65-lexAop). (H,H′) Glial cells (green) in PB and CA regions. Magenta, Repo staining. Repo∩T2 indicates the presence of type II lineage glia. (I,I′) Glial cell distributions after inducing apoptosis in all type II lineage glia (Repo∩T2; grim). (J) Quantification of glial cell numbers. n=14 and 10, respectively. Scale bars: 50 μm.