Fig. 4.

Blocking cell death in Repo-positive cells or entire lineages reveals lineage-characteristic phenotypes. (A) Genetics for blocking apoptosis in type II lineages. (B-K′) Composite confocal images of adult fly brains carrying various CLIn clones of type II lineages. All the offspring of the CLIn clones are labeled in magenta, and the offspring positive for repo-GAL4 are also marked in green for morphology and in blue for nuclei (lacZ). The clone identity and the transgene used to block apoptosis in Repo-positive offspring (UAS-p35) or entire progeny (lexAop-p35) are indicated. The dashed boxes in D-K are shown at higher magnification on the right (D′-K′). (B-D′) Increase of DM5 glia (B,B′) by either UAS-p35 (C,C′) or lexAop-p35 expression (D,D′). (E-G′) The DM6 glial cells (E,E′) are increased after lexAop-p35 (G,G′), but not UAS-p35, expression (F,F′). (H,H′) Glial cells from DM5-INP1 (right hemisphere). (I-K′) The DM6-INP1 do not contain glial cells (I,I′). Blocking apoptosis by lexAop-p35 rescued some glia (K,K′), while blocking apoptosis by UAS-p35 fails to rescue any glia (J,J′). In H,I, brains were contaminated with a DM5 NB clone and a type I lineage clone, respectively. Scale bars: 50 μm. See also Figs S4, S5 and S6.