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. 2018 Jun 14;145(12):dev159012. doi: 10.1242/dev.159012

Fig. 3.

Fig. 3.

Normal turning of type II peripheral axons is disrupted in PCP mutants. (A,B) Whole-mount NF200 immunolabeling of the type II peripheral axons reveals stereotyped turns towards the cochlear base in the more mature basal (A) and less mature apical (B) regions of the E18.5 mouse cochlea. (C) The morphology of individual type I and type II neurons is also revealed by sparse DiI labeling. (D-F) In the Vangl2 KO cochlea, incorrectly turned axons (arrowheads) projecting towards the cochlear apex can be identified using both NF200 (D,E) and DiI (F). Similar turning errors occur in the basal region of Fzd3 KOs (G) at E18.5 and in Celsr1 KOs (H) at P0. (I) Quantification of averaged turning error frequencies in Vangl2 (n=3), Fzd3 (n=3), Celsr1 (n=3) and Celsr3 (n=3) KO mice relative to littermate controls (n=3 for each). Data are mean±s.e.m. *P<0.05, **P<0.01 for KOs versus littermate controls (Student's t-test). Scale bars: 10 µm.