Fig. 3.

Representative Spectra Comparing the Enhanced RNA Extraction Method to the Conventional Extraction Method.

RNA was extracted from six independent NRVM samples using either the conventional RNA extraction protocol (old-red) or the enhanced protocol (new-blue). (A) The spectra were recorded with a NanoDrop 2000 and overlayed for comparison within and between groups. Dashed line appears over 270 nm in the old protocol (red) and 260 nm in the new protocol (blue). (B) Box and whisker plots (min to max) of RNA concentrations based on the absorption spectra in RNA samples extracted using the old protocol versus the new protocol. Asterisk (*) denotes a significant difference between groups, unpaired T-test (*p < 0.05), n = 6 old, n = 6 new.