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. 2018 Jul 6;7(7):52. doi: 10.1038/s41389-018-0061-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Wound-healing assay was performed in control (Scr) and FOXC1-silenced (siFOXC1) HPAC cells; migration was analyzed using an automated Nikon Biostation CT at 2 h intervals for up to 96 h at × 4 magnification. b Quantitative analysis of migration in FOXC1-silenced HPAC cells was calculated using NIS-Element AR software. c Wound-healing assay was performed in control (empty vector) and FOXC1-overexpressing (OE FOXC1) MIA PaCa-2 cells; migration was analyzed using an automated Nikon Biostation CT at 2 h intervals for up to 96 h at × 4 magnification. d Quantitative analysis of migration in FOXC1-overexpressing MIA PaCa-2 was calculated using NIS-Element AR software. e Invasiveness of FOXC1-silenced HPAC cells was observed using a Matrigel invasion assay (left: × 20 magnification; right: × 100 magnification). f Percentage invasion of control (Scr) and FOXC1-silenced (siFOXC1) HPAC cells was obtained by counting invaded cells from each group. g Invasiveness of FOXC1-overexpressing MIA PaCa-2 cells was observed using a Matrigel invasion assay (left: × 20 magnification; right: × 100 magnification). h Percentage invasion of control (pCMV) and FOXC1-overexpressing (OE FOXC1) MIA PaCa-2 cells was obtained by counting invaded cells from each group. i Colony formation assay was performed with control (Scr) and FOXC1-silenced (siFOXC1) HPAC cells (left: × 20 magnification; right: × 100 magnification). j The percentage of colonies were calculated with parental HPAC control cells serving as the baseline. k Colony formation assay was performed with control (pCMV) and FOXC1-overexpressing (OE FOXC1) MIA PaCa-2 cells (left: × 20 magnification; right: × 100 magnification). l The percentage of colonies were calculated with parental MIA PaCa-2 control cells serving as the baseline. m Western blot analysis of EMT markers in FOXC1-silenced HPAC cells and n FOXC1-overexpressing MIA PaCa-2 cells. Data shown as mean ± SEM. Experiments (n = 3) were repeated three times in triplicates. *p < 0.05