Streptomyces sp. SMS_SU21 possesses strong antimicrobial activity and antioxidant potential.
ABSTRACT
Streptomyces sp. SMS_SU21 possesses strong antimicrobial activity and antioxidant potential. This strain was isolated from the Sundarbans mangrove ecosystem, and its draft genome comprises 7,449,420 bp with 6,680 open reading frames. Genome analysis of strain SMS_SU21 provides insight into its secondary metabolite arsenal and reveals the gene clusters putatively responsible for its bioactive potential.
GENOME ANNOUNCEMENT
Mangrove streptomycetes are rich sources of natural products with significant biological activities and novel structures (1). Genome mining of mangrove streptomycetes accelerates the rapid discovery of useful products originating from them. In this study, Streptomyces sp. SMS_SU21 was isolated from the soil sediment of the Sundarbans mangrove ecosystem in India. This strain possesses potent antimicrobial activity against a broad spectrum of microorganisms, including multidrug-resistant strains and various phytopathogens (2). Interspecies competition within the residing microbial population is an obvious phenomenon in the Sundarbans, due to the rich index of species diversity and limited consumable nutrient sources (3) found there. Thus, in-depth information regarding the genomic edifice of this strain is required to understand its survival strategies in a competitive environment like the Sundarbans mangrove ecosystem.
Genomic DNA was extracted with a HiPurA streptomycetes DNA isolation and purification kit (Himedia, India). Shotgun sequencing was performed with a high-throughput HiSeq platform (Illumina) at AgriGenome Labs Private Limited in Kerala, India. Prior to whole-genome analysis, Cutadapt version 1.8 (4) was used to remove adapter sequences, and all low-quality data (Q < 30) were filtered out using Sickle version 1.33 (5). The cleaned reads were subjected to analysis with Kmergenie (6) to predict the optimal k-value and assembly size, which were found to be 31 and 7,449,420 bp, respectively. De novo assembly was performed using SPAdes version 3.9.0 (7), Velvet (8), and QUAST (9). The genome sequence of Streptomyces sp. SMS_SU21 was annotated using the Rapid Annotations using Subsystems Technology (RAST) server (10) and the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) version 4.3 (11). The draft genome sequence of Streptomyces sp. SMS_SU21 constituted a total of 93 contigs (>1,000 bp), with a total size of 7,449,420 bp and a G+C content of 72.3%. The RAST server predicted 6,680 coding sequences, of which 2,208 (34%) were annotated as SEED subsystem features and 4,472 (66%) were annotated as outside the SEED subsystem; 3 rRNAs and 67 tRNAs were also predicted. The closest related type strains based on the 16S rRNA gene sequence are S. griseorubens NBRC 12780 (GenBank accession number AB184139), S. althioticus NRRL B-3981 (GenBank accession number AY999791), and S. griseoincarnatus LMG 19316 (GenBank accession number AJ781321), all with 99% sequence identity.
Secondary metabolite biosynthetic gene clusters (BGCs) were predicted using antiSMASH version 4.0 (12), which identified 24 putative BGCs in the genome. This includes nonribosomal peptide synthetase (NRPS) gene clusters, polyketide synthase (PKS), novel hybrid PKS-NRPS gene clusters, and other BGCs for producing siderophores, lantipeptides, lassopeptide, and bacteriocin. Numerous genes responsible for resistance to toxic compounds, including arsenic, mercury, cobalt, tellurium, and cadmium, were additionally detected. Hence, Streptomyces sp. SMS_SU21 may have great potential to produce exclusive bioactive natural compounds for clinical, industrial, and environmental applications.
Accession number(s).
This whole-genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession number PNRA00000000. The version described in this paper is the second version, PNRA02000000.
ACKNOWLEDGMENTS
We thank the World Bank, the ICZM project (54-ICZMP/3P), and DST-SERB for providing financial and instrumental support. Sohan Sengupta acknowledges UGC for providing his fellowship. Arnab Pramanik is supported by a research associateship from the ICZM project. Pijush Basak thanks the Science and Engineering Research Board (SERB), New Delhi, India, for providing the financial assistance in the form of grants and fellowships (project file number YSS/2015/001123/LS).
We are also grateful for the use of the instrument facility provided by UGC-CAS, DST-FIST, DBT-IPLS at the Department of Biochemistry, University of Calcutta, India, and to the local people of the Sundarbans for their moral support and active cooperation.
Footnotes
Citation Sengupta S, Pramanik A, Basak P, Bhattacharyya M. 2018. Draft genome sequence of bioactive strain Streptomyces sp. SMS_SU21, isolated from soil sediment of the Sundarbans mangrove ecosystem. Genome Announc 6:e00614-18. https://doi.org/10.1128/genomeA.00614-18.
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