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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Oncogene. 2018 Apr 9;37(27):3740–3752. doi: 10.1038/s41388-018-0206-3

Figure 3.

Figure 3

TP53 mutations in patient #5: Left panels illustrate read alignments using Integrative Genomics Viewer. Right panels show the percentage of alternative reads found in each compartment [i.e., tissue (top), PBMC (middle), plasma (bottom)]. Two adjacent mutations of TP53, located 3 base pairs apart, with a variant allele fraction of 80% were detected in tissue. The corresponding alternative alleles of these 2 mutations were not detected in PBMC, thus excluding germline events. Ultra-deep sequencing of plasma DNA detected the same 2 mutations in approximately 4% of circulating DNA fragments. Mutations in plasma are found in the exact same reads, identifying their shared origin from their tissue counterparts.