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. 2018 May 24;8(13):3461–3473. doi: 10.7150/thno.25179

Figure 3.

Figure 3

Fluorescence spectrum assay under different experimental conditions, including FAM-labeled DNA probe, FAM-labeled DNA probe + nanoquenchers, FAM-labeled DNA probe + target p53 gene + nanoquenchers, and nanoquenchers, including MOF alone (A), MOF@GO (B), MOF@AuNP (C), and MOF@AuNP@GO (D). (E) The corresponding fluorescence intensity under different experimental conditions from A to D. The concentrations of FAM-labeled DNA probe and target p53 gene in the final solution are 1 nM and 20 nM, respectively; and the concentrations of four MOF-based nanoquenchers of MOF alone, MOF@GO, MOF@AuNP, and MOF@AuNP@GO in the final solution are 5, 3.75, 2 and 2 µg mL-1, respectively. Fluorescence spectra were collected with the excitation of 485 nm and the emission of 516 nm. Error bars were obtained from three independent repeat experiments.