Figure 3.
Evaluation of immune response to probiotic treatment. (A) Pro-(IL-1β and TNF-α) and anti-(IL-10) inflammatory cytokines levels have been determined in vaginal washes of mice, treated intravaginally with 10 μl of Saline, or L. crispatus (2 × 109/ml) or GI (108/ml) and infected with G. vaginalis (5 × 107/20 μl/mouse), at days +1 and +3 post-infection. Data are the mean ± SEM from 2 independent experiments each with 6 mice/group. (B) Histological inflammation was assessed by haematoxylin-eosin staining of formalin-fixed, paraffin-embedded vaginal tissue sections. Images (Bar = 200 µm, Magnification 10x) are representative of 2 separate experiments with similar results.