Table 1. Yeast strains used in this study.
All strains are derived from WL001 and WL002. All Ire1 constructs listed below are 3x FLAG-tagged within their lumenal domains.
| Strain | Species | Description |
|---|---|---|
| yWL001 | Sc | WT, mat A, leu2-3,112 TRP1 can1-100 ura3-1 ADE2 his3-11,15 |
| yWL002 | Sc | ire1∆::NATR |
| yWL003 | Sc | ire1∆::NATR, HO::Sp IRE1 |
| yWL004 | Sc | ire1∆::NATR, HO::SpLumScCyto IRE1 |
| yWL005 | Sc | ire1∆::NATR, HO::ScLumSpCyto IRE1 |
| yWL006 | Sc | ire1∆::NATR, HO::Sc IRE1 |
| yWL007 | Sc | ire1∆::NATR, HO::ScLum/transmembrane/linkerSpKR IRE1 |
| yWL008 | Sc | WT, leu2::5'hac1-gfp-3'hac1 |
| yWL009 | Sc | ire1∆::NATR, leu2::5'hac1-gfp-3'hac1 |
| yWL010 | Sc | ire1∆::NATR, leu2::5'hac1-gfp-3'hac1, HO::Sp IRE1 |
| yWL011 | Sc | ire1∆::NATR, leu2::5'hac1-gfp-3'hac1, HO:: SpLumScCyto IRE1 |
| yWL012 | Sc | ire1∆::NATR, leu2::5'hac1-gfp-3'hac1, HO:: ScLumSpCyto IRE1 |
| yWL013 | Sc | ire1∆::NATR, leu2::5'hac1-gfp-3'hac1, HO::Sc IRE1 |
| yWL014 | Sc | leu2::5'hac1-gfp-3'hac1, his3::pTdh3-mCherry |
| yWL015 | Sp | WT, mat h+, ade6-M210, ura4-D18, leu1-32 |
| yWL016 | Sp | ire1∆::KANR |
| yWL017 | Sp | ire1∆::KANR, leu1::Sp IRE1 |
| yWL018 | Sp | ire1∆::KANR, leu1::SpLumScCyto IRE1 |
| yWL019 | Sp | ire1∆::KANR, leu1::ScLumSpCyto IRE1 |
| yWL020 | Sp | ire1∆::KANR, leu1::Sc IRE1 |
| yWL021 | Sp | ire1∆::KANR, leu1::SpLumScCyto IRE1, ura4::5'hac1-gfp-3'hac1 |
| yWL022 | Sp | ire1∆::KANR, leu1::ScLumSpCyto IRE1, ura4::5'hac1-gfp-3'hac1 |
| yWL023 | Sp | ire1∆::KANR, leu1::Sc IRE1, ura4::5'hac1-gfp-3'hac1 |
| yWL024 | Sp | bip1::bip1-hac1 hybrid |
| yWL025 | Sp | bip1::bip1-hac1 hybrid A->U |
| yWL026 | Sp | bip1::bip1 splicing variant |
| yWL027 | Sp | ura4::synthetic splicing substrate |