Video 4. Motion correction of KPC Src-FRET biosensor cells and liver autofluorescence imaged in an intrasplenic experiment through optical window.

Download video file ^{(6.9MB, mp4)}

DOI: 10.7554/eLife.35800.018

Associated with Figure 5D. (top left) Each intensity frame from the FLIM acquisition (green) superimposed on the reference frame (magenta). (top right) The motion compensated frames (green) superimposed on the reference frame (magenta); frames which have been excluded due to a poor correlation are marked as such. (bottom left) Cumulative intensity merged FLIM lifetime image without motion correction, estimated using the first moment of the decay. (bottom right) Cumulative intensity merged FLIM lifetime image with motion correction, estimated using the first moment of the decay.