Figure 6. eled expression precedes that of germline-specific nanos-1.

(A) Double FISH of eled and nanos-1 in 3-week juveniles, showing the emergence of nanos-1 expression in gonadal primordia. (B) Magnified boxed gonadal regions in (A). Images are numbered from less to more mature worms. Images are confocal sections. Empty arrowheads: eled⁺nanos-1^-; solid arrowheads: eled⁺nanos-1⁺. (C) Quantification of nanos-1 expression in eled⁺ presumptive germ cells as a function of worm length. Symbols represent cell counts in individual animals. Note the sharp transition at worm length ~400 µm (N = 13).

Figure 6—figure supplement 1. nanos-1 expression is germline specific.

WISH of (A) nanos-1 and (B) nanos-2 in juveniles (top: male; bottom: female). While nanos-2 is expressed in both ε and δ’-cells, the other schistosome nanos homolog, nanos-1, only shows expression in testes, ovary, and vitellaria.