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. 2018 Jun 19;7:e34519. doi: 10.7554/eLife.34519

Figure 1. Pancreatic islet innervation in zebrafish is established early in development and maintained in juvenile and adult stages.

Figure 1.

(A-D) Whole mount immunostaining of wild-type zebrafish at 50, 75, 100, 120 hr post fertilization (hpf) for acetylated Tubulin (nerves), Insulin (beta cells), Somatostatin (delta cells), and DAPI (DNA). (E–F) Whole mount immunostaining of 25 days post fertilization (dpf) zebrafish and 1 year zebrafish pancreas and intestine following tissue clearing with the CLARITY protocol. Maximum intensity projections are presented; A, anterior; D, dorsal; V, vagus nerve; ND, nodose ganglion; P, pancreas; S, sympathetic innervation; Pi, intra-pancreatic innervation.