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. 2018 Jun 6;4(6):e00642. doi: 10.1016/j.heliyon.2018.e00642

Fig. 5.

Fig. 5

Effect of stem cell derived EVs on T cell proliferation. EVs were isolated from the conditioned medium of CMPC and BM-MSC to investigate their immunomodulative potential. A. Fluorescence microscope visualization of the EV uptake by T cells. Red: EVs (PKH-26). Green: T cells (CFSE). Blue: nucleus (Hoechst). B Titration curve of CMPC derived EV protein concentration and the effect on T cell proliferation after stimulation. A cut-off point is reached around 1 μg of EV protein. C BM-MSC- and CMPC-derived EVs significantly reduce T cell proliferation (BM-MSC-EV: 73 ± 12%, CMPC-EV: 77 ± 10%). **p < 0.01 compared to stimulated T cell.