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. Author manuscript; available in PMC: 2018 Jul 25.
Published in final edited form as: Biochemistry. 2017 Jul 10;56(29):3708–3709. doi: 10.1021/acs.biochem.7b00493

Figure 4.

Figure 4

(A) HPLC analysis of the RutA reaction run in the absence of NADH showing the formation of a new compound at 20.3 min. (B) Co-elution of the new compound with synthetically prepared FMN-N5-oxide. (C) Comparison of the UV−vis spectra of FMN and FMN-N5-oxide formed in the RutA full reaction. (D) Extracted ion chromatograms for [M + H]+ = 473.1 Da demonstrate FMN-N5-oxide formation only in the full reaction mixture. (E) The exact mass of the new compound is consistent with the mass expected for the FMN-N5-oxide ([M + H]+ = 473.1 Da). (F) Exact mass of the new compound formed when the reaction is performed in the presence of 18O2.